中文摘要：

建立了高效液相色谱-串联质谱法（HPLC-MS／MS）同时测定人体尿液中游离酪氨酸和色氨酸分析新方法。采用乙腈－0．1％甲酸溶液（10：90，V：V）为流动相，流速为0．3 mL·min－1。在全扫描模式下，将m／z为182．2、205．2的离子分别确定为酪氨酸和色氨酸的母离子，m／z136．1与188．0分别为定量子离子。在质谱的多反应监测正离子模式下进行分析，酪氨酸和色氨酸的检测限分别为0．10和0．25 ng·mL－1。在已优化的色谱和质谱条件下，待测样品经过简单的离心、过滤后即可直接进样检测。在3个不同添加水平的回收实验中，酪氨酸和色氨酸的平均回收率为74．0％～124．0％，RSD值小于7．97％，表明该方法可满足实际样品检测要求。该方法操作简单、快速、灵敏度高，可用于人体尿液中游离酪氨酸和色氨酸的同时测定。

英文摘要：

A new method for the simultaneous determination of free tyrosine and tryptophan in human urine samples by HPLC-MS/MS was developed.The chromatographic separation was achieved on an Agilent XDB-C18 column（4.6×50 mm,1.8μm）,the column temperature was 25 ℃,the mobile phase was CH3CN-0.1%HCOOH（10：90,v/v）,the flow rate was 0.3 mL·min-1 .In the full scan mode,the m/z 182.2 and m/z 205.2 were identified as the precursor ions of tyrosine and tryptophan,the m/z 136.1 and m/z 188 were quantitative product ion,respectively.The analysis was realized by multiple reaction-monitoring modes.Under optimal conditions,the linear ranges of tyrosine and tryptophan were 0.001-2.00μg · mL-1 ,and the limits of detection were 0.10 and 0.25 ng·mL-1 ,respectively.The average recoveries for tyrosine and tryptophan（spiked at three different levels）ranged from 74.0 to 124.0%,the RSD was below 7.97%.The method is simple,rapid and sensitive,and suitable for the simultaneous determination of free tyrosine and tryptophan in human urine.