中文摘要：

目的研究组蛋白去乙酰化酶（HDACs）抑制剂西达本胺对胃癌细胞株体外抗增殖作用及其可能机制。方法用西达本胺终浓度为0、16、32、64、128、256μmol/L（分别为B、A1、A2、A3、A4、A5组）处理SGC-7901细胞24、48、72h。采用MTT法及流式细胞术分别检测细胞增殖及细胞凋亡,反转录PCR（RT-PCR）检测沉默信息调节因子2相关酶1（SIRT1）及HDAC11mRNA表达,实时荧光定量PCR（RT-qPCR）检测表皮生长因子受体（EGFR）mRNA表达,Western blot检测Notch1蛋白的表达。结果与B组相比,西达本胺呈浓度依赖性地抑制SGC-7901细胞增殖（P〈0.05）,提高SGC-7901细胞凋亡率（P〈0.05）,阻断SIRT1、HDAC11、EGFR mRNA以及Notch1蛋白表达（P〈0.05）。结论西达本胺可抑制SGC-7901细胞增殖,促进细胞凋亡;其作用机制可能是降低SIRT1、HDAC11基因的表达或与Notch及EGFR信号通路相关。

英文摘要：

Objective To investigate the antiproliferative effect and underlying mechanism ofhistone deacetylase（HDAC） inhibitor chidamide on human gastric cancer cell line SGC-7901 in vitro. Methods SGC-7901 cells were divided into six groups of B （ehidamide 0 μmol/L）, A1 （chidamide 16μmol/L）, A2（ehidamide 32 μmol/L）, Aa（chidamide 64 μmol/L）, A4（ehidamide 128 μmol/L） and A5 （chidamide 256 μmol/L）. The cell proliferation and cell apoptosis were detected by MTT and flowcytometry, respectively. The mRNA expressions of silent mating type information regulator 2 homolog 1（SIRT1） and HDACll were determined by reverse transcription-PCR（RT-PCR）, and the mRNAexpression of epithelial growth factor receptor（EGFR） was measured by real-time quantitative-PCR （RT-qPCR）. The protein expression of Notch1 was analyzed by Western blot. Results Comparedwith group B, chidamide inhibited cell proliferation and promoted cell apoptosis in a dose-dependent manner（P〈0. 05）, and significantly blocked the expressions of SIRT1 mRNA, HDACll mRNA,EGFR mRNA and Notch1 protein （P〈0. 05 ）. Conclusion Chidamide can inhibit SGC-7901 cell proliferation and enhance cell apoptosis probably via decreasing gene expressions of SIRT1 andHDAC11 or correlating with Notch and EGFR signaling pathway.