中文摘要：

目的探讨人单核源性巨噬细胞中Tribbles（TRBs）的表达及氧化低密度脂蛋白（ox-LDL）对TRBs（TRB1、TRB2、TRB3）表达的调节。方法体外培养人单核源性巨噬细胞，应用ox-LDL处理自然分化的人单核源性巨噬细胞，采用Real—TimePCR检测人单核源性巨噬细胞TRBsrnRNA的表达情况及其不同浓度的ox-LDL、低密度脂蛋白（LDL）处理不同的时间后三种TRBsmRNA的表达。结栗人单核源性巨噬细胞中，TRBs的表达以TRB3为主，TRB1、TRB2微量表达；ox-LDL能促进TRB2和TRB3表达，减低TRB1表达。与LDL对照组相比，50μg／mLOX—LDL刺激巨噬细胞后，TRBImRNA的表达减少70％（P〈0．01），TRB2mRNA的表达增加5．2倍（P〈0．01），TRB3mRNA增加3．5倍（P〈0．05），并且这种调节作用均呈剂量和时间依赖性。结论本研究证实了TRBs基因在人单核源性巨噬细胞中有表达，OX-LDL能调节巨噬细胞TRBsmRNA的表达，推测TRBs特别是TRB3可能参与了OX-LDL诱导的巨噬细胞凋亡过程。

英文摘要：

Objective To investigate the expression of Tribbles （TRBs） in human monocyte-driven macrophages and to analyze the regulative role of oxidized low density lipopmtein （ox-LDL） on TRB1, TRB2 and TRB3. Methods Primary human mono- cyte-driven macrophages were cultured in vitro. Macrophages were incubated with ox-LDL and low density lipoprotein （LDL） at various concentrations for various times. The level of TRBs mRNA was determined by quantification real-time RT-PCR. Results All three TRBs genes （TRB1, TRB2 and TRB3） were expressed in human monocyte-driven macrophages, among them, TRB3 was predominant, and TRB1 and TRB2 were little expressed. Ox-LDL increased the levels of TRB3 andTRB2 mRNA, while it decreased the expression of TRB1 in a dose- and time-dependent manner. Conclusions All three TRBs are expressed in human monocyte-driven macmphages and can be regulated by ox-LDL, so TRBs, especially TRB3 may participate in the course of mac- rophage apoptosis.