中文摘要：

目的：检测基质细胞衍生因子1（SDF-1）及其受体CXCR4 mRNA和蛋白在子宫内膜癌细胞系及组织中的表达。方法：应用免疫组织化学和RT—PCR技术，检测子宫内膜癌细胞系Ishikawa和HEC-1A细胞，44例子宫内膜癌组织，26例非典型增生子宫内膜、单纯增生子宫内膜和正常子宫内膜组织中SDF-1和CXCR4 mRNA和蛋白表达水平。结果：HEC-1A和Ishikawa细胞均表达CXCR4 mRNA；Ishikawa细胞中SDF-1 mRNA弱表达，而HEC-1A细胞中SDF-1 mRNA未见表达。CXCR4和SDF-1在正常和良性病变的子宫内膜组织腺体细胞中的阳性表达率为100％，在子宫内膜癌组织的腺体细胞中的阳性表达率分别为90．9％和93．2％。各种子宫内膜组织中间质细胞CXCR4和SDF-1的表达均显著弱于腺体细胞。在子宫内膜癌腺体细胞和间质细胞中CXCR4和SDF-1的表达均显著弱于正常及良性病变者。在低分化的子宫内膜癌腺体细胞中CXCR4和SDF-1的表达弱于高分化者。结论：CXCR4和SDF-1在子宫内膜癌细胞系、子宫内膜组织及内膜癌组织中的表达存在差异，在内膜癌组织中其表达均显著弱于正常及良性病变者。

英文摘要：

Objective： To investigate the expressions of SDF-1 and CXCR4 in human endometrial carcinoma tissues. Methods： SDF-1 and CXCR4 protein expression levels were detected by immunohistochemical staining in 26 cases of atypic hyperplasia endometrium, simple hyperplasia endometrium and normal endometrium and 44 cases of endometrial carcinoma tissues. Results： Both SDF-1 mRNA and CXCR4 mRNA were expressed in Ishikawa, while no expression of SDF-1 mRNA was detected in HEC- 1A. The rates of CXCR4 and SDF-1 protein expressions were 100% in glandular cells of human normal endometrial tissues, while 90.9% and 93.2% in glandular cells of endometrial carcinoma tissues. These immunoreactivities of SDF-1 and CXCR4 were significantly low in endometrial carcinoma tissues as compared with those in normal endometrium;and the intensities of immunohistochemical staining significantly decreasesd in immunoreactivity of SDF-1 and CXCR4 in low grade as compared with that in hight grade. Conclusion： SDF-1 and CXCR4 were expressed differently in endometrial cell lines, human normal endometrial tissues, and endometrial carcinoma tissues. As compared with normal endometrium , these were significantly low levels expression of SDF-1 and CXCR4 in endometrial carcinoma tissues.