中文摘要：

通过雄激素“浸泡法”诱导尼罗罗非鱼Oreochromis niloticus仔鱼雄性化的研究，探索一种雄激素浸泡诱导尼罗罗非鱼仔鱼雄性化的技术方法。选用了可芳化的MT（17α-methyhesosterone）和不可芳化的MDHT（17α-methyldihydrotestosterone）两种雄激素进行对比试验。进行了处理时期（1，7，13DPH，Days Post Hactch-ing），激素剂量（200，600，1800，5400μg·L^-1），持续时间（2，4，8h）3个因素的简单比较法正交试验。结果如下：当诱导7DPH仔鱼、持续时间为4h时，MT剂量为600μg·L^-1时雄性率最高，为98．81％，而剂量1800，5400μg·L^-1时雄性率明显下降（P〈0．05）；MDHT剂量为600，1800μg·L^-1时，雄性率最高，分别为98．34％和98．39％，剂量为200，5400μg·L^-1时雄性率没有明显下降（P〉0．05）。当用MT诱导7DPH仔鱼，剂量为600μg·L^-1时，持续时间为4h的雄性率最高、而8h的雄性率明显下降（P〈0．05）；当用MDHT时，3种持续时间（2，4，8h）的雄性率无明显差异（P〉0．05）。总体来看，应用MT和MDHT的雄性率都能达到生产应用需求，但MDHT效果更稳定、表现出更好的应用潜力。

英文摘要：

In this paper, we concentrated on masculinization of larvae Nile Tilapia （Oreochromis nilotic- us） by immersion the larvae in androgens MT （17α-methyhestosterone） or MDHT （17α-methyl- dihydrotestosterone）. The three factors were considered： treatment period （1, 7, 13 DPH, Days Post Hatching）, hormone dose （200, 600, 1 800, 5 400 μg·L^-1）, and treatment duration （2, 4, 8 h）. All these three factors were taken into account by simple comparison orthogonal experimental design. The main results are as follows ： the 7 DPH larvae induced by MT at a dose of 600 μg·L^-1 with 4 h treatm duration is the best result of masculinization, male rate is 98.81% , while at a dose of 1 800 μg·L^-1 5400 μg·L^-1, male rate greatly decreased （P〈0.05）; 600 μg·L^-1 or 1 800 μg·L^-1 dose of MDHT present the best results of masculinization, male rate is 98.34% and 98.39% respectively, at a dose of 200 μg·L^-1 or 5 400 μg·L^-1 male rate are not significantly decreased （ P 〉 0. 05 ） . When 7 DPH larvae were induced by MT at a dose of 600 μg·L^-1, 4 h is the best treatment duration, 8 h pres- ents greatly decreased result （P 〈 0.05） ; When MDHT was used, three treatment durations （2, 4, 8 h） got consistent results （P 〉 0. 05 ）. The conclusion is that MT and MDHT can both achieve masculin- ization goals for application of production, but MDHT is more stable and show a better application value.