中文摘要：

目的：研究DHA/EPA比例对高脂膳食小鼠肝脏氧化应激和炎症因子表达的影响。方法：将40只雄性apo E-/-小鼠随机分为4组并给予高脂膳食,同时用葵花籽油、紫苏油、鱼油和藻油等调节膳食中n6/n3 PUFA的比例为4：1或DHA/EPA的比例（2：1、1：1和1：2）。12周后,用商业试剂盒或实时荧光定量PCR法检测小鼠血清或肝脏组织的氧化应激水平或炎症因子表达变化。结果：DHA/EPA替代植物来源的n3 PUFA-ALA并不能减少血清脂质过氧化产物MDA生成及提高血清和肝脏的SOD活性;与补充ALA相比,DHA/EPA为1：1时小鼠血清GSH含量显著升高（P〈0.05）,DHA/EPA比例为1：1和1：2时小鼠肝脏的GSH水平显著升高（P〈0.05）;DHA/EPA比例2：1可显著降低血清及肝脏的促炎因子表达。结论：在高脂膳食及膳食n6/n3 PUFA比例4：1的条件下,补充DHA/EPA比植物来源的ALA更能提高抗氧化能力和降低炎症水平,且当DHA/EPA比例1：1时抗氧化能力最好,而当DHA/EPA比例2：1时抗炎能力最强。

英文摘要：

Objective：To investigate the ratios of DHA/EPA on oxidative stress and inflammatory factors in mice treated with high-fat diets.Methods：Forty male apo E-/-mice were randomly divided into 4 groups and were given high-fat diets.Meanwhile,sunflower seed,perilla,fish,and algal oils were used for adjusting the proportion of n6/n3 PUFA 4：1 and DHA/EPA（2：1,1：1,and 1：2）.After 12 weeks feeding,commercial kits and/or real-time PCR were used to test the redox status and inflammatory factors expression in mice serum and/or liver.Resuts：Replacement of ALA with DHA and EPA could not decreaseserum MDA production and improve serum and hepatic SOD activity.Compare to ALA group,serum GSH levels were increased significantly（P〈0.05） in DHA/EPA 1：1 group.The levels of GSH in serum and liver were both improved（P〈0.05） in DHA/EPA 1：1 and 1：2 groups.The expression of pro-inflammatory factors in serum and liver was reduced（P〈0.05） in DHA/EPA 2：1 group.Conclusion：Supplementation with DHA/EPA was superior to ALA when consumed high-fat diets with n6/n3 PUFA ratio 4：1,since the antioxidant ability of DHA/EPA 1：1 and the anti-inflammatory capacity of DHA/EPA 2：1 are more powerful than ALA.