中文摘要：

本文利用RT—PCR和PCR技术制备地高辛标记的谷胱甘肽过氧化物酶（ATGPX3）cDNA探针，分别进行DNA和RNA斑点和印迹杂交分析．通过调整杂交液组分的浓度和增加10％硫酸葡聚糖的方法，改进杂交反应．实验结果表明，改良的方法不仅提高了杂交效率，而且明显检测到RNA杂交印迹反应．另外，利用地高辛标记cDNA探针技术也检测到了植物激素ABA诱导的ATGPX3基因的表达，同时证明了该方法可以用来检测植物基因的表达．

英文摘要：

In this work, ATGPX3 cDNA probe labelled with digoxigenin was prepared by using RT-PCR and PCR methods. A series of DNA and RNA were detected by dot blot hybridization analysis. In order to improve hybridization reaction, the paper changed concentrations of some constituents of hybridization buffer and added 10 Dextran Sulphate. The results showed that the improved protocol not only raised the hybridization efficiency, but also obviously detected signal by RNA blot hybridization analysis. Moreover, ABA-induced ATGPX3 gene expression was also detected using digoxigenin-labelled cDNA probe, and this method could be used to detect the gene expression in plants.