中文摘要：

本文从哥伦比亚生态型的拟南芥（Arabidopsis thaliana Columbia0）中克隆了谷胱甘肽过氧化物酶AtG—PX3的完整编码序列（Coding sequence，CDS）以及部分片段的CDS序列，构建了该基因的不同区域的表达载体，在Transetta（DE3）的BL21大肠杆菌中表达，并成功纯化了GPX3膜蛋白，不仅为后续实验研究GPX3的结构与功能提供了材料，而且为研究膜蛋白表达与纯化的方法提供了借鉴．

英文摘要：

In this study, the author cloned the whole coding sequence and some fragments of GPX3 from Arabidopsis thaliana ecotype Columbia, and constructed them to some expression vectors, and then transformed the vectors into Escherichia coli strain Rosetta （DE3） and the GPX3 was purified successfully. The work not only provides materials for the further study of the structure and function of the GPX3, but also offers a method for expressing and purifying the membrane proteins.