中文摘要：

将发光试剂钌（Ⅱ）联吡啶固定在离子交换树脂上,在酸性高锰酸钾氧化条件下,用于测定人血清中的雷尼替丁（RAN）。使用BDS C18柱为固定相,流动相为甲醇-磷酸盐缓冲溶液（pH 3.0）（35：65,V/V）。雷尼替丁的质量浓度在2.0×10-4～4.0×10-2mg/mL范围内呈良好的线性,回归方程为ΔICL=15.016ρ＋187.09（r=0.9981）。检出限（3σ）为67 ng/mL。对2.0μg/mL的雷尼替丁进行11次平行测定,其相对标准偏差为2.2%。

英文摘要：

A novel method was developed for the determination of ranitidine by high performance liquid chromatography（HPLC） with chemiluminescence（CL） detection.The chromatographic separation was performed on a Hypersil BDS-C18 column（i.d.： 250 mm×4.6 mm,particle size： 5 μm,pore size： 100） with an isocratic mobile phase consisting of methanol and 0.02 mol/L KH2PO4（pH 3.0）（volume ratio 35：65） at a flow rate of 1.0 mL/min.The detection was based on the chemiluminescence reaction of ranitidine with acidic potassium permanganate（KMnO4） and tris（2,2（-bipyridyl）ruthenium（III）,which was immobilized on the cationic ion-exchange resin.Under optimum conditions,the linear range for ranitidine was 2.0×10-4～4.0×10-2 mg/mL with the detection limit of 67 ng/mL（3σ）.The relative standard deviation（RSD） was 2.2%（n=11） for 2.0 μg/mL ranitidine.Ranitidine in the serum samples was analyzed by the proposed method with satisfactory results.