中文摘要：

选用28日龄断奶仔猪10头,分别在其颈动脉、肝门静脉和肠系膜静脉安装插管,待仔猪恢复1周后,通过肠系膜静脉连续灌注1%对氨基马尿酸测定仔猪采食后0~6 h内门静脉的血流速度。结果显示,体质量为9.97 kg的仔猪在采食前的血流速度为1.78 L.kg-1.h-1或295 mL/min,采食后1 h内血流速度快速上升,比采食前的血流速度提高了23.6%,1~5 h内血流速度的变化趋于平缓,6 h血流速度达到最高（2.52 L.kg-1.h-1或419mL/min）;10头试猪中的6头可成功用于肠道营养物质代谢研究。本试验建立的仔猪颈动脉-门静脉-肠系膜静脉插管系统为肠道营养物质代谢的研究提供了技术支持。

英文摘要：

The study was aimed to establish techniques for implanting arterial and portal catheters in piglets used to investigate intestinal nutrient metabolism.Ten 28-day-old piglets were surgically implanted with catheters in one carotid artery,the portal vein,and the mesenteric vein.After 7 d recovery period,a 1% solution of p-amino hippurate（pAH） was infused continuously into the mesenteric catheters to determine portal plasma flow（PPF） within 0 to 6 h after the start of feeding.The results showed that PPF of piglets（9.97 kg bodyweight） rapidly increased within 1 h after the start of feeding,increased by 23.6% compared to that（1.78 L·kg-1·h-1 or 295 mL/min） determined before the feeding,and reached the peak（2.52 L·kg-1·h-1 or 419 mL/min） at 6 h after a gradual increase during 1 to 5 h post-feeding;6 of 10 pigs could be used for the following research on intestinal nutrient metabolism.These results indicated that the established techniques for implanting catheters in the carotid artery,the portal vein,and the mesenteric vein in piglets provide a technique support for the study of intestinal nutrient metabolism.