中文摘要：

目的：建立同时测定己烯雌酚（diethylstilbestrol,DES）和辛基酚（octylphenol,OP）的电化学方法。方法：通过自组装技术将氧化石墨烯（graphene oxide,GO）组装到壳聚糖修饰的玻碳电极表面,构建GO-壳聚糖修饰玻碳电极,用循环伏安法和差分脉冲伏安法研究DES和OP在该修饰电极上的电化学行为及其测定,利用Origin8软件多峰曲线拟合分析实验数据。结果：在p H 7.0磷酸盐缓冲溶液中,同时测定DES和OP的线性范围分别为3.31×10-7--1.24×10-5 mol/L和2.89×10-7--9.50×10-6mol/L,检测限为7.56×10-8 mol/L。且该修饰电极具有良好的重复性和稳定性。结论：GO/壳聚糖修饰玻碳电极测定酚类环境刺激素具有简单、快速等优点,可同时测定DES和OP的混合样品。

英文摘要：

Objective： To establish an electrochemical determination of diethylstilbestrol（DES） and octylphenol（OP） at the same time. Methods ： Through self- assembly methods, graphene oxide（ GO） was assembled to chitosan modified glassy carbon electrode surface by selfassembly to build the GO-chitosan modified glassy carbon electrodes, in phosphate buffer solution of p H 7.0, we used the method of cyclic voltammetry and differential pulse voltammetry to study the method and admeasurement of electrochemical action and determine DES and OP on the modified electrodes. Origin8 software multi-peak curve fitting analysis was applied to analyse the data of experiment. Results： In the solution of p H 7.0, the linearity range of DES and OP were 3.31 ×10-7-1.24 ×10-5mol/L and 2.89 ×10-7-9.50 ×10-6mol/L. Detection limit is 7.56 ×10-8mol/L, it was indicated that modified electrode had good repeatability and stability. Conclusion： Determination of phenolic environment estrogen in GO-chitosan modified glassy carbon electrodes is simple but high-speed, can test the composite sample of DES and OP.