中文摘要：

旨在研究山羊瘤胃产甲烷古菌的多样性并与其他动物瘤胃进行比较。采用产甲烷菌特异性引物Met86F／Metl340R研究山羊瘤胃产甲烷古菌16SrRNA基因的多样性，随机挑选100个克隆进行序列分析。结果表明，所有克隆经限制性内切酶酶切后共获得16个OTU，绝大多数为甲烷短杆菌，其中与甲烷短杆菌菌株AK-87、ZA-10、OCP、SM9和30Y序列最相近的克隆数分别为58％、19％、7％、2％和2％，还有与Methanos phaera stadtmanae（1％）、Metha-nobacterium aarhusense（2％）、Acidulipro fundum boonei（3％）、Methanobrevibacter sp．AK-87（4％）和Methanobrevibactersp．1Y（2％）等相似的古菌序列。将研究结果与已经报道的关于牛和绵羊瘤胃产甲烷菌多样性比较，发现不同PCR引物可检测出不同菌群结构，而饲料类型、动物种类可影响瘤胃产甲烷菌的菌群结构。

英文摘要：

This experiment was conducted to study the molecular diversity of rumen methanogens from goat and its comparison with other ruminant. The methanogen-specific primers Met86F/ Met1340R was used to analyze the 16S rRNA gene diversity of methanogens. One hundred clones were randomly picked up for sequence analysis. The result showed that sixteen OTUs were ob- tained after RFLP analysis, and the majority belonged to the Methanobrevibacter strains, AK-87 （58%）, and others to ZA-10 （19%）, OCP （7%）, SM9 （2%）, and 30Y（2%）, and Methano- sphaera stadtmanae-like （1%） , Methanobacterium aarhusense-like （2%） , aciduli pro f undum boonei-like （3 %）, Methanobrevibacter sp. AK-87-1ike （4%） and Methanobrevibacter sp. 1Y-like （2%） sequences, respectively. With comparison to ported in previous studies, the rumen can be affected it can be concluded that by the different primers, rumen methanogens from other ruminants re- the structure of methanogenic communities in feed types and animal species.