中文摘要：

目的研究成釉细胞瘤（ameloblastoma，AB）中hTERT启动子区的DNA甲基化，并探讨其生物学意义。方法选取新鲜标本AB12例，同时用11例正常黏膜做对照观察，用甲基化特异PCR检测上述组织中人类端粒酶反转录酶（human telomerase reverse transcripase，hTERT）启动子区的DNA甲基化。结果AB、正常黏膜中hTERT启动子区的DNA非甲基化阳性分别为4例（4／12）和6例（6／11）。AB、正常黏膜中hTERT启动子区的DNA甲基化阳性分别为11例（11／12）和3例（3／11），其中4例AB和1例正常黏膜同时表现hTERT启动子区DNA的甲基化和非甲基化。结论AB的hTERT启动子区的DNA甲基化比正常黏膜常见且有意义；hTERT启动子的甲基化可能对hTERT基因起调节作用。

英文摘要：

Objective To study the DNA methylation of human telomerase reverse transcripase （hTERT） promoter in amelobalstoma （AB） and investigate its clinical biological significance. Methods DNA methylation of hTERT promoter in 12 cases of AB and 11 cases of normal oral mucosa were detected by methylation-specific PCR. Results The positive cases of unmethylated hTERT promoter region in AB and normal oral mucosa were 4 （4/12） and 6 （ 6/11 ）, respectively （ P 〈 0. 001 ）. The positive cases of methylated hTERT promoter region in AB and normal oral mucosa were 11 （ 11/12 ） and 3 （ 3/11 ）, respectively. Four cases of AB and 1 case of normal oral mucosa showed both DNA methylation and unmethylation of hTERT promoter region in the same time. Conclusions DNA methylation of of hTERT promoter region in AB was more common than that in normal oral mucosa. The DNA methylation in hTERT promoter maybe regulate hTERT gene expression.