目的:建立高效液相色谱方法测定天山地区红景天属植物根中红景天苷、苷元酪醇和百脉根苷的含量。方法:液相色谱分离系统采用岛津VP5.0溶剂输送系统,LC10A TVP自动进样器,PDA检测器,色谱柱VP—ODS 4μm,150mm×4.5mm,流动相为甲醇-乙腈-水(25:5:70),柱温40℃,分析时间15min,检测波长275nm,进样量20μL。结果:方法简单、灵敏和精确。3物质的线性范围分别为:0.1714—2.2282mg·mL^-1(r=0.9985),0.2012-2.6156mg·mL^-1(r=0.9996)和0.1922~2.4986mg·mL^-1(r=0.9994);回归方程分别为Y=1.885×10^4+5.247×10^5 X,Y=-1.284×10^4+7.077×10^5X和Y=1.076×10^4+4.132×10^5X。结论:发现Rhodiolarosa rosea L.根中含有(44.38±0.7)mg·g^-1红景天苷,(16.37±0.9)mg·g^-1苷元酪醇和(8.06±0.7)mg·g^-1百脉根苷,并生长于相对较低海拔高度,认为是一个较具开发利用前景的目标植物。
Objective:To establish an HPLC method to determine the contents of salidroside, tyrosol and lotaustralin in roots of Rhodiola L. plants grown at Tianshan mountain. Method : The HPLC system consisted of Shimadzu Class VPS. 0 multi -solvent delivery system;Shimadzu LC 10A TVP auto sampler; Shimadzu LC 10A TVP photodiode array detector( PDA), Shimadzu temperature control module. Column : Shimadzu VP - ODS 4μm, 150 mm × 4.5 mm. Solvent : isocratic with methanol, acetonitrile and water ( 25: 5 : 70 ). Flow : 1.0 mL · min^ - 1 at 40℃. Analysis time : 15 min. Detection :at 275 nm with injection volume 20μL. Results :The method was simple, sensitive and accurate. The linearity of method were observed at the ranges from 0. 1714 to 2. 2282 mg· mL^-1 ,0. 2012 to 2. 6156 mg· mL^-1 and 0. 1922 to 2.4986 mg· mL^-1 ;regression equations were Y= 1. 885 × 10^4 +5. 247×10^5X (r =0.9985), Y = - 1. 284 × 10^4 + 7. 077 × 10^5X( r = 0.9996)and Y = - 1. 076 × 10^4 + 4. 132 × 10^5X ( r = 0.9994 ) respectively. Conclusion:It is found that Rhodiola rosea L. containing(44.38 ±0.7)mg· g^-1 sahdroside, (16.37±0.9)mg·g^-1 tyrosol and (8.06±0.7) mg· g^- 1 lotaustralin, further utilization research. growing at lower altitude, is considered as the target plant for further utilization research.