通过对低强度激光照射离体人红细胞后的生物物理学指标分析,找出影响红细胞(RBC)生物特性的主要因素。以放置在4℃下48h的变形性下降的正常人血液为样品研究低强度激光对红细胞流变学特性的影响。取2mL标本分成相等两份,其中一份在2mW,4mW,8mW的功率下照射30min,另一份离心后取出红细胞放入等渗的PBS液中用同样的方法进行照射。照射后进行对比发现单照红细胞的样品的变形性、取向、膜流动性、微黏度、各向异性和渗透脆性比全血照射下的变化小。表明红细胞膜的生物放大效应对血液的流变学特性的影响是主要的。
This paper studied the biophysics' index of low power laser irradiation on human's red blood cell (RBC) to find the primary factor that affects biophysics of RBC. The blood cells of the deformability health human deposited in 4 ℃ for 48 h are used as samples, and the effects of low power laser irradiation on these samples are investigated. These samples are divided into two groups. One group is irradiated for 30 min under the laser with the powers of 2 mW, 4 mW, and 8 mW. The RBCs in the other group are doped with PBS with the same concentration. After centrifugating, they are processed in the same way. The results show that the characteristics of the blood cell irradiated by laser are less than the whole blood. Our study indicates that the magnify effects of the human's red cells' membrane are important to the rheology characteristic of the blood.