牛病毒性腹泻病毒(Bovineviraldiarrheavirus,BVDV)有两种基因型:基因1型(BVDV-1)和基因2型(BVDV-2)。以往的流行病学调查显示BVDV-1的分布和流行区域更加广泛,但近年来国内BVDV-2的发病率呈持续升高趋势。BVDV-2通常会引起严重的急性感染和出血综合征,给养牛业造成巨大损失。本文就BVDV-2特异性检测方法和基因分型的建立,型特异性BVDV-2毒株的分离与鉴定,BVDV-2优势分离株的全基因组测定与遗传特性分析及BVDV-1和BVDV-2抗原和抗体检测平台的建立等方面展开简要综述。
Bovine viral diarrhea virus is divided into two genotypes:genotype1(BVDV-1)and genotype2(BVDV-2).Previous epidemiological survey showed BVDV-1isolates distributed more widely.However,the mobidity rate of BVDV-2is increasing in recent years.In general,BVDV-2infection can cause more severe disease characterized by acute infection and haemorrhagic syndrome,causing enormous losses to the cattle industry.In this review,we dilate on the following aspects:the establishment of the specifi c detection and genotyping methods of BVDV-2,the isolation and identifi cation of BVDV-2,complete genomic sequencing and phylogenetic analyzation of BVDV-2isolates,and the establishment of detection platform to virus and antibody of BVDV-1and BVDV-2.