中文摘要：

目的观察X射线照射对培养星形胶质细胞（astrocyte,AS）增殖和分泌的影响。方法建立体外培养大鼠纯化的AS物理损伤模型（划痕损伤模型）,分为对照组、划痕组和放疗组。利用免疫荧光观察胶质细胞胶质纤维酸性蛋白（glial fibrillary acidic protein,GFAP）和5-溴脱氧尿嘧啶核苷（5-bromodeoxy uridine,BrdU）的表达,利用RT-PCR观察细胞因子IL-6和TNF-a的表达。结果划痕损伤刺激能使培养AS反应性增生活化。损伤后6h出现IL-6、TNF-a表达水平增高,24h后损伤周围BrdU阳性细胞率明显增加。通过X射线照射能抑制AS的BrdU表达,但并不能抑制损伤后IL-6和TNF-a的表达。结论X射线照射可以通过调控细胞周期,有效抑制损伤后AS的反应性增生,但不能对活化AS的IL-6和TNF-a的表达起到抑制作用。

英文摘要：

Objective To observe the effect of X-ray on the proliferation and secretion of cultured astrocytes （AS）. Methods The model of mechanical injury to cultured purified AS was established. The cultured cells were randomly divided into 3 equal groups： control group,scratch group （scratch ＋ vehicle） and irradiation group （scratch ＋ irradiation）. Immunofluorescent （IF） staining was used to detect the expression of glial fibriliary acidic protein （GFAP） and 5-bromodeoxyuridine （BrdU）. RT-PCR was used to detect the mRNA level of IL-6 and TNF-a. Results The ＂scratch-wound injury＂ induced AS proliferation and activation. At 6h after scratch injury,the mRNA level of IL-6 and TNF-a increased. At 24h and 48h after scratch injury,the percent of BrdU-positive cells increased significantly. X-ray decreased the percent of BrdU-positive cells, but did not inhibit the expression of IL-6 and TNF-a. Conclusion In response to injury,AS enter a cell cycle and show reactive gliosis. X-ray can inhibit injury-induced reactive gliosis,but can not inhibit the secretion of IL-6 and TNF-a by reactive AS.