中文摘要：

目的：研究糖尿病视网膜病变患者内皮祖细胞（EPCs）的数量变化,从而探讨内皮祖细胞是否参与了糖尿病微血管病并发症的发生发展。方法：选择与年龄、性别相匹配的患者,共124例,其中健康对照组62例,单纯糖尿病组31例（DM组）,糖尿病背景期视网膜病变15例（DM＋NPDR组）,糖尿病增殖期视网膜病变组16例（DM＋PDR组）。采用密度梯度离心法从人外周血分离出单个核细胞,通过流式细胞仪检测内皮祖细胞数量。结果：与健康对照组相比,DM组、DM＋NPDR组、DM＋PDR组的外周血EPCs的数量明显减少（P〈0.05）。DM＋NPDR组与DM组相比,外周血EPCs数量无统计学差异（P〉0.05）。DM＋PDR组与DM组相比,外周血EPCs数量显著增加（P〈0.05）。结论：EPCs参与了糖尿病微血管并发症的发生发展,有望在临床治疗中成为潜在的治疗靶点。

英文摘要：

Objective： To study the number of endothelial progenitor cells（EPCs） from patients with diabetic retinopathy, so as to explore whether endothelial progenitor cells（EPCs） participate in the development of diabetic microangiopathy complication.Methods： Choose the patients of matching with age and sex, in total include 124 ases, with 62 cases in healthy control group, simple diabetes group 31 cases（DM group）, 15 cases with background diabetic retinopathy（DM＋NPDP group）, 16 cases with the proliferatiive diabetic retinopath（DM ＋ PDR）. Using density gradient centrifugation, isolated from human peripheral blood mononuclear cells,the number of endothelial progenitor cells was detected through the flow cytometry. Results： Compared with healthy controls, the number of peripheral blood was decreased significantly patients with the DM group, the DM ＋ NPDR group, and the DM ＋ PDR group（P 〈 0.05）.DM ＋ NPDR group compared with DM group, the number of peripheral blood EPCs no statistical difference（P 〉 0.05）. DM ＋ PDR group compared with DM group, the number of peripheral blood EPCs increased significantly（P 〈 0.05）. Conclusion： EPCs are involved in the development of diabetic microvascular complications, is expected to become potential therapeutic targets in the clinical treatment.