中文摘要：

模拟人体的生理环境，用荧光光谱法，紫外可见光谱法，循环伏安法探究了高三尖杉酯碱（HHRT）与DNA的反应。结果表明高三尖杉酯碱与DNA的反应是嵌入反应。普通的化学分析方法不能解析出化学反应中每种物质的纯光谱，本文应用多元曲线分辨．交替最小二乘（McR—ALS）方法解析了HHRT与DNA的竞争反应所获得的光谱。通过光谱解析，获得了所有组分的纯光谱和浓度分布曲线，结果令人满意。由MCR-ALS计算得到的HHRT与DNA的结合常数和化学计量系数分别为5．2×10^4L／mol和1。

英文摘要：

The interaction of homobarringtonie （HHRT） with DNA under pseudo-physiological conditions （Tris-HCl buffer of pH 7.4） was investigated with the use of spectrofluorimetry, UV vis spectrometry and viscosity measurement. The experiment results suggested that the HHRT binds to DNA with an intercalation mode. However, traditional chemical methods couldn＇t obtain the pure spectra and concentration profiles of the all constituents. With the aid of chemometrics approach, multivariate curve resolution-alternating least squares （MCR-ALS）, which was applied to resolve the spectral data matrix of the interaction between HHRT and DNA, the pure spectra and concentration profiles was obtained with satisfactory results. The binding constant and stoichiometric coefficient of HHRT to DNA were 5.2x104 L/tool and 1 resnectivelv calculated bv MCR-ALS.