利用分光光度法(UV)研究了不同摩尔比的Cu2+与槲皮素的配合物生成过程,并采用多元分辨-交替最小二乘法(MCR-ALS)对实验数据进行分析.结果表明在中性条件下,Cu2+与槲皮素只生成一种最终产物(吸收峰值为295nm),中间经历了吸收峰值为438nm的过渡态.MCR-ALS分辨的结果为Cu2+-槲皮素配合物的形成机理提供了理论依据.
The reaction process of Cu2+ and quercetin with different molar ratio were studied in this paper using ultraviolet-visible absorption spectroscopy(UV).Experimental data were analyzed by multivariate curve resolution-alternating least squares(MCR-ALS).It was shown that there were two products,intermediate product with the absorption peak at 438 nm and final product with the absorption peak at 295 nm.