中文摘要：

目的 观察α-硫辛酸对高糖环境下INS-1细胞的抗氧化作用及对胰岛素分泌的影响,探讨α-硫辛酸对胰岛β细胞的保护作用。方法 INS-1细胞分为3组：对照组（11.1 mmol/L葡萄糖处理）、高糖组（30 mmol/L葡萄糖处理）和观察组（30 mmol/L葡萄糖＋200μmol/Lα-硫辛酸处理）。分组培养48 h后检测活性氧生成的情况,行葡萄糖刺激胰岛素分泌试验（GSIS）测定胰岛素含量,测定各组氧化应激相关指标,测定核因子E2相关因子2（Nrf2）、血红素加氧酶1（HO-1）、醌氧化还原酶1（NQO1）mRNA表达情况。结果 高糖组细胞存活率、超氧化物歧化酶（SOD）、谷胱甘肽过氧化酶1（GPX-1）和过氧化氢酶（CAT）含量低于对照组（P〈0.05）,活性氧和丙二醛含量高于对照组（P〈0.05）。观察组细胞存活率、SOD、GPX-1和CAT含量高于高糖组（P〈0.05）,活性氧和丙二醛含量低于高糖组（P〈0.05）。高糖组基础胰岛素分泌和GSIS试验胰岛素分泌量以及SOD、GPX-1、CAT、HO-1和NQO1 mRNA表达低于对照组（P〈0.05）。观察组基础胰岛素分泌和GSIS试验胰岛素分泌量以及SOD、GPX-1、CAT、Nrf2、HO-1和NQO1mRNA表达高于高糖组（P〈0.05）。结论 α-硫辛酸显著降低胰岛β细胞在高糖环境下的氧化损伤,增强胰岛β细胞GSIS的功能,对胰岛β细胞具有保护作用。

英文摘要：

Objective To observe effects of α-lipoic acid on anti-oxidation of INS-1 cells and insulin secretion under high glucose environment, and to investigate protective effect of α-lipoic acid on pancreatic β cells. Methods INS-1 cells were divided into control group （11.1 mmol/L glucose treatment） , high glucose group （30 mmol/L glucose treatment） and observation group （30 mmol/L glucose ＋ 200 μmol/L α-lipoic acid treatment）. After 48 h of cultiva- tion, production conditions of reactive oxygen were detected, glucose-stimulated insulin secretion （GSIS） assay was per- formed to detect insulin concentration. Related indexes of oxidative stress, and mRNA expressions of nuclear factor E2- related factor 2 （ Nrf2）, heme oxygenase-1 （HO-1）, NAD （P） H quinone oxidoreductase （NQO1） were also detected. Results In high glucose group, values of cell survival rate, and contents of superoxide dismutase （ SOD ） , glutathione peroxidase 1 （GPX-1） and catalase （CAT） were significantly lower （P 〈 0. 05 ） , while reactive oxygen species and malondialdehyde contents were significantly higher than those in control group （P 〈 0.05）. In observation group, values of cell survival rate, SOD, GPX-1 and CAT contents were significantly higher （P 〈 0. 05） , while reactive oxygen species and malondialdehyde contents were significantly lower than those in high glucose group （P 〈 0. 05 ）. Values of basal insu- lin secretion, insulin secretion in GSIS assay, and mRNA expressions of SOD, GPX-1, CAT, HO-1 and NQO1 in high glucose group were significantly lower than those in control group （P 〈 0. 05）. Values of basal insulin secretion, insulin secretion in GSIS assay, and mRNA expressions of SOD, GPX-1, CAT, Nrf2, HO-1 and NQO1 in observation group were significantly higher than those in high glucose group（ P 〈 0.05 ）. Conclusion The α-lipoic acid can significantly reduce oxidative damage of islet 13 cells under high glucose environment and enhance GSIS function of