以芽变毛白杨幼嫩枝条为外植体进行愈伤组织和茎段组织培养试验研究。结果表明:芽变毛白杨诱导愈伤组织培养基为:MS+6-BA2.0~4.0mg/L+2,4-D1.5~2.0mg/L+NAA0.25mg/L+KT2.0~4.0mg/L+3%蔗糖,愈伤组织分化芽培养基为MS+6-BA0.5mg/L+NAA0.5mg/L+KT 2.0mg/L+3%蔗糖时,培养效果好。茎段培养时,适宜无根苗生长的培养基为MS+6-BA 0.5mg/L+NAA0.1mg/L+3%蔗糖,生根诱导适宜的培养基为1/2MS+IBA0.5~1.0ms/L+1.5%蔗糖。
Induction of callus and stems of the mutant Populus tomentosa was studied.The results indicated the medium for callus induction was MS + 6 - BA 2.0 ~ 4.0 mg/L + 2,4 - D 1.5 ~ 2.0 mg/L+ NAA 0.25 mg/L+ KT 2.0 mg/L+ 3% sucrose;the best differentiation medium was MS + 6 - BA 0.5 mg/L + NAA 0.5 mg/L + KT 0.5 mg/L + 3% sucrose; the best medium for stem was MS + 6 - BA 0.5 mg/L + NAA 0.5 mg/L + KT 2.0 mg/L + 3% sucrose; the medium fed rooting induction was 1/2MS+ IBA 0.5 ~ 1.0 mg/L+ 1.5% sucrose.