中文摘要：

在 ischemic 织物的 Angiogenesis 是建筑群和多基因事件。在学习，我们构造了 hypoxic 反应元素(HRE ) ，在 Tet 上推进了双 controlled 系统并且在暴露于组织缺氧和 pharmacologic 正式就职的老鼠 cardiomyocytes 在 hVEGF165 和 angiopoietin-1 (Ang-1 ) 基因的表示上调查了他们的效果。我们与 recombinant rAAV-rtTA-Rs-M2/rAAV-TRE-Tight-Ang-1 和 rAAV-9HRE-hVEGF165 感染了新生的老鼠 cardiomyocytes。我们的结果显示病毒的 titer 是 1 宴 ????? 崥吗？？

英文摘要：

Angiogenesis in ischemic tissue is a complex and multi-gene event. In the study, we constructed hypoxic re-sponse elements （HRE） and the Tet-On advanced double-controlled systems and investigated their effects on the expression of hVEGF165 and angiopoietin-1 （Ang-1） genes in rat cardiomyocytes exposed to hypoxia and pharma-cologic induction. We infected neonatal rat cardiomyocytes with recombinant rAAV-rtTA-Rs-M2/rAAV-TRE-Tight-Ang-1 and rAAV-9HRE- hVEGF165. Our results indicated that the viral titer was 1×1012 vg /mL and the viral purity exceeded 98%. hVEGF165 expression was induced by hypoxia, but not by normoxia （P 0.001）. Ang-1 expression was evident under doxycycline induction, but undetectable without doxycycline induction （P 0.001）. Immunofluorescence staining showed that positively stained hVEGF165 and Ang-1 protein appeared only under both hypoxia and doxycycline induction. We demonstrate here that HRE and the recombinant Tet-On advanced double gene-controlled systems sensitively regulate the expression of hVEGF165 and Ang-1 genes in an altered oxygen environment and under pharmacological induction in vitro.