中文摘要：

目的：探究湿阻中焦证动物模型以及藿香正气散的干预对葡萄糖代谢糖异生途径的影响，揭示湿阻中焦证动物模型对葡萄糖代谢糖异生途径的影响的相关机制，从代谢组学层面揭示脾胃参与调控能量代谢以及藿香正气散燥湿和胃的科学内涵。方法：参照前期较成熟的湿阻中焦证动物模型的造模方法，自然造模与综合造模相结合，模拟湿病产生的三大主要因素，建立湿阻中焦证模型。检测湿阻中焦证模型及藿香正气散干预后的大鼠肝脏葡萄糖、丙酮酸、丙酮酸羧化酶、葡萄糖-6-磷酸酶的含量变化。结果：与空白组比较，湿阻中焦证模型丙酮酸含量降低，丙酮酸羧化酶、葡萄糖-6-磷酸酶、肝脏葡萄糖含量均增加；藿香正气散能增强葡萄糖代谢糖异生作用。结论：造模前后，肝脏葡萄糖含量增加，这可能是因为湿阻中焦证会导致能量消耗增加，而机体对葡萄糖的吸收和利用不足。同时这可能是湿阻中焦证身倦乏力、食少纳差的病理基础之一；藿香正气散对湿阻中焦证模型的糖异生有增强作用，且对葡萄糖的分解、转化有一定的作用，这可能是藿香正气散治疗湿病，缓解其身倦乏力、食少纳差等症状的作用机制之一。

英文摘要：

Objective： Research of Huoxiangzhengqi powder intervention on syndrome of dampness in middle Jiao model effects on metabolism glucose and gluconeogenesis mechanism to reveal the effects of syndrome of dampness in middle Jiao model on glucose me- tabolism in the gluconeogenic pathway, revealed in the regulation of energy metabolism and the spleen and stomach Huoxiangzhengqi powder in the treatment of syndrome of dampness in middle Jiao of the scientific connotation from the level of system biology. Methods ： The natural model and comprehensive model combined with the three main factors of establishing simulation of dampness, syndrome of dampness in middle Jiao model. Measure the content of pyruvate pyruvate carboxylase and glucose by biochemical method ; measure the content of pyruvic acid and glucose-6-phosphatase by enzyme-linked immunosorbent assay. Results： The content of syndrome of damp- ness in middle Jiao model of pyruvic acid reduce, pyruvate carboxylase, glucose-6-phosphatase are reduce and glucose content in- creased; Huoxiangzhengqi powder can enhance the glucose metabolism of gluconeogenesis. Conclusion： Thesyndrome of dampness in middle Jiao will lead to increased energy consumptionrather than the body~ absorption and use of glucose lead to inadequate energy sup- ply, which may be one of the pathological basis of syndrome of dampness in middle Jiao of lassitude, anorexia, diet reduction ; Huox- iangzhengqi powder could strengthen the effect of syndrome of dampness in middle Jiao energizer gluconeogenesis and glucose some of the decomposition and transformation, which may be Huoxiangzhengqi powder treatment of rheumatism, a mechanism of action to alle- viate the lassitude, anorexia and other symptoms of diet reduction.