中文摘要：

目的：探讨胃癌中血小板反应蛋白2（ THBS2）的表达及其临床意义。方法在14对配对胃癌样本及其正常对照中，使用实时荧光定量PCR和Western blot法检测THBS2 mRNA和蛋白表达水平。使用129例胃癌样本构成的组织芯片评价THBS2的临床意义。生存分析使用Kaplan-Meier法和Cox比例风险模型。结果 THBS2 mRNA和蛋白表达在14对样本中有12例胃癌样本中都显著低于其正常对照。与Western blot结果一致，组织芯片中胃癌THBS2表达明显低于正常对照（P=0．031）。高表达的THBS2与患者的良好预后密切相关（ P=0．002），并且THBS2表达越低胃癌的组织分化程度越低（ P =0．005）。结论与正常胃组织相比THBS2在胃癌组织中表达下调，可能在胃癌的发生发展中起到重要的作用，并且可作为一个潜在的胃癌临床预后因子。

英文摘要：

Objective To explore the expression of THBS 2 and its clinical significance in gastric cancer . Methods＆amp;nbsp;The mRNA and protein expression levels of THBS2 were assessed in 14 paired of gastric cancer specimen and corresponding normal mucosa using quantitative real-time PCR and Western blot analysis. Immunohistochemistry of TH-BS2 on a population-based tissue microarray consisting of 129 gastric cancer cases was used for evaluating the TH-BS2 prognostic significance. Kaplan-Meier method and Cox′s proportional hazards model were used in survival analysis. Results Both mRNA and protein expression of THBS2 in 12 gastric cancer tissues were remarkably lower than the corresponding normal tissues among total 14 pair tissues. Consistent with the results of our Western blot, THBS2 expression was significantly inhibited in gastric cancer tissues as that in the normal controls in TMA （ P=0. 031） . Overexpression of THBS2 had a significant correlation with favorable prognosis of gastric cancer patients （ P=0. 002 ） and decreasing THBS2 expression was associated with the poor histological grade of gastric cancer histological grade （ P=0. 005 ） . Conclusion This study suggests THBS2 is down-regulation in gastric cancer tissue versus normal gastric tissue, and it may play a critical role in gastric cancer carcinogenesis and may be a potential prognosis predictor of gastric cancer.