中文摘要：

目的 观察脂多糖（lipopolysaccharide,LPS）损伤大鼠黑质（substantia nigra,SN）后迷走神经背核（dorsal motor nucleus of the vagus,DMV）中酪氨酸羟化酶（tyrosine hydroxylase,TH）和多巴胺受体1（dopamine receptor 1,DR1）和多巴胺受体2（dopamine receptor 2,DR2）的表达变化。方法 30只SD大鼠根据随机数字表分为LPS大鼠组和对照组。LPS大鼠组双侧SN内注射LPS,对照组双侧SN内注射等体积的生理盐水。6周后进行转棒实验,检测胃排空能力,免疫荧光和免疫印迹检测SN内TH和DMV内TH、DR1和DR2的表达。结果 经鉴定LPS组大鼠造模成功,胃排空能力降低,DMV中TH阳性细胞数量从（7±3）个增加到（12±2）个（P〈0.05）,DR1阳性细胞数量从（28±5）个减少到（18±6）个（P〈0.05）,DR2阳性细胞数量从（20±3）个增加到（33±4）个（P〈0.05）。背侧延髓内,TH蛋白表达水平从（0.16±0.02）增多到（0.43±0.04）（P〈0.05）,DR1的蛋白表达水平从（0.78±0.05）下降至（0.36±0.03）（P〈0.05）,DR2的蛋白表达水平从（0.43±0.03）增加至（0.58±0.07）（P〈0.05）。结论 LPS大鼠DMV中TH、DR1和DR2表达变化可能与帕金森病患者的胃排空障碍存在一定的联系。

英文摘要：

Objective To observe the expression of tyrosine hydroxylase （TH）、dopamine receptor 1 （DR1） and dopamine receptor 2 （DR2） in rat dorsal motor nucleus of vagus （DMV） induced by substantia nigra （SN） lesion by lipopolysaccharide （LPS）. Methods According to the random number table, a total of 30 female SD rats were randomly divided into a LPS group and a normal control group. LPS was injected into the bilateral SN of the rats in the LPS group, and the same volume of saline was injected into the same position of the rats in the normal control group. After six weeks, rotating rod experiment, gastric emptying, immunofluorescent assay and western blotting analysis were used to detect TH expression in the SN and TH, DR1 and DR2 expression in the DMV. Results Six weeks later,the LPS group rats were identified successfully, and the gastric emptying ability was reduced, the number of TH immunoreactive neurons in the DMV of the LPS group increased from （7±3） to （12±2） （P〈0.05）, the number of DR1 immunoreactive neurons decreased from （28 ± 5） to （18 ± 6） （P〈0.05） and the number of DR2 immunoreactive neurons increased from （20±3） to （33±4） （P〈0.05）. In the dorsal medulla, the level of TH protein increased from （0.16±0.02） to （0.43± 0.04） （P〈0.05）, the level of DR1 protein decreased from （0.78±0.05） to （0.36±0.03） （P〈0.05） and the level of DR2 protein increased from （0.43 ± 0.03） to （0.58 ± 0.07） （P〈0.05）. Conclusion The changes of TH, DR1 and DR2 expression in DMV of LPS group rats may be associated with gastric emptying dysfunction in patients with Parkinson＇s disease.