中文摘要：

目的探讨实时定量测定干扰素诱导基因[抗黏病毒1（MXl）基因，2’5，-寡腺苷酸合成酶1（OASl），干扰素诱导蛋白44（IFl44）基因]表达水平与系统性红斑狼疮（SLE）临床表现及病情活动度的相关陛。方法收集100例SLE患者，40例非SLE其他自身免疫性疾病患者，40名健康对照的临床资料，取外周血抽提总RNA并反转录成eDNA，运用SYBRgreendyeI实时定量聚合酶链反应（QT—PCR）在ABIPRISM7000基因测序仪上检测患者和对照组的MXl、OASl和IFl44定量表达水平（以ACT值表示）的差异，并与各临床指标及病情活动度进行相关性分析。采用方差分析和Spearman相关分析进行统计学处理。结果①SLE患者总体的MXl、OASl、IFl44mRNAACT值（3．4±1．8，4．20．1．5，8．8±2．2）明显高于非SLE患者组（2．4±0．4，3．4±0．7，5．4±2．1）和健康对照组（2．3±1．2，2．6±0．7，5．2±2．O）；②SLE患者组的OAS1、IFl44mRNA△CT值在不同活动度的SLE组内日乍活动组（3．8±1．4，3．2±1．8）；轻度活动组（4．8±1．5，8．0±2．2）；重度活动组（6．0±1．4，12．1±2．4）]差异具有统计学意义；③SLE患者组的OASl、IFl44mRNAACT值与SLE疾病活动指数（SLEDAI）积分之间有相关性（r=O．338，0．380）；④在有关节炎的SLE患者中，其MXl、OASl、IFl44表达水平升高，与无关节炎患者比较差异具有统计学意义；⑤在狼疮肾炎组内，IFl44mRNAACT值表达（2．2±1．1）与非狼疮肾炎组（3．2±2．1）比较差异有统计学意义；⑥SLE组内3个基因表达存在高相关性且具有统计学意义（P均〈0．05）。结论干扰素诱导基因MXl、OASl、IFl44在SLE患者中均有表达上调现象，OASl、IFl44mRNA实时定量表达水平对SLE患者的病情活动度判断有意义，且三者在SLE的发生发展中亦存在密切联系。

英文摘要：

Objective To investigate the expression of the genes correlated with interferon induced genes virus （MX1, OAS1, IFI44） in the peripheral blood leukocytes of patients with systemic lupus erythematosus （SLE）, and to evaluate the relationships between the expression levels of these genes and disease activity. Methods The clinical data of 100 SLE patients, 40 non-SLE patients with rheumatic diseases, and 40 normal controls were collected. Peripheral blood samples were collected. Total RNA was extracted and transcribed into cDNA. SYBR green dye based real-time quantitative PCR method was used to compare the expression levels （indicated as ACT value） of MX1, OAS1 and IFI44 in patients with SLE and those in the controls. Comparisons between groups were performed with ANOVA and Spearman correlations. Results （1） The ACT value of MX1, OAS1 and IFI44 expression level of the SLE patients （3.4±1.8, 4.2±1.5; 8.8±2.2） was significantly higher than those of the non-SLE patients （2.4±0.4, 3.4±0.7, 5.4±2.1 ） and normal controls （2.3±1.1, 2.6±0.7, 5.2±2.0）. （2） The ACT value of OAS1 and IF144 expression level of the SLE patients in severe disease was significantly higher than those of the SLE patients in mild disease and the SLE patients with stable disease. （3）The ACT value of OAS1 and IFI44 were correlated with the SLEDAI scores （r=0.038, 0.380）. （4） The ACT values of MX1, OAS1 and IFI44 expression level of the SLE patients with arthritis were significantly higher than those of SLE patients without arthritis. （5） The ACT value of IFI44 expression level of the SLE patients with lupus nephritis （3.2±2.1,2.2±1.1） was significantly higher than that of the SLE patients without lupus nephritis. （6） There was correlations among these genes in SLE patients （P〈0.05）. Conclusion The value of MX1, OAS1 and IFI44 expression level of SLE patients is up-regulated. The real time expression levels of OAS1, IFI44 genes are associated with SLE disease activity and there are