中文摘要：

目的：研究17β-雌二醇（E2）对人自然杀伤细胞株NK92中干扰素γ（IFN-γ）分泌的影响及其可能的机制。方法：用E2和poly I：C处理NK92细胞,分别在4、8、12和24小时后用实时定量PCR、酶联免疫吸附法和流式细胞术测定NK92细胞中IFN-γ的表达情况,用实时定量PCR检测microRNA-29b（miR-29b）的变化。进一步,构建含有IFN-γ3＇UTR的pEGFP-C1质粒,并转染pre-29b和pEGFP-IFN-γ3＇UTR质粒至HEK293A细胞中,用流式细胞术检测绿色荧光蛋白（GFP）的变化。结果：E2抑制NK92细胞的IFN-γ分泌和上调miR-29b的表达;miR-29b能够直接靶向调节IFN-γ3＇UTR mRNA。结论：E2可能通过调节NK92的miR-29b而影响IFN-γ的分泌水平,进而影响其免疫功能。

英文摘要：

Objective：To investigate the possible mechanism on the effects of 17β-Estradiol（E2） on human natural killer cell line NK92.Methods：The NK92 were treated with E2 and poly I：C for different time（4,8,12 and 24 hours）.The expression of IFN-γ was measured by qRT-PCR,ELISA and FCM.The expression of miR-29b was determined by qRT-PCR.Meanwhile,the recombinant plasmid pEGFP-IFN-γ 3′ UTR,the pre-29b and pEGFP-IFN-γ 3′ UTR plasmid were constructed and transfected into HEK293A cell by lipofectamine 2000.The expression of GFP was detected by FCM.Results： E2 could down-regulate the secretion of IFN-γ and up-regulate the expression of miR-29b by NK92.Furthermore,miR-29b could modulate the expression of IFN-γ 3′ UTR mRNA directly.Conclusion：The effect of E2 on the immune function of NK cell may be attributed to the secretion of IFN-γ via modulating miR-29b expression.