中文摘要：

根据牙釉质（amelogenin,AMEL）同源基因在山羊X、Y染色体上存在不同程度碱基缺失的特点,设计1对引物,对山羊血液基因组DNA混合样进行PCR扩增,将PCR产物纯化、克隆并测序;然后对46个山羊血液DNA样本（♀：22个,♂：24个）进行性别鉴定。结果显示,雌性山羊样品经扩增只产生一条非特异性条带,雄性山羊样品产生一条非特异性条带和一条特异性条带;非特异性片段长度为349 bp,特异性片段为289 bp;46个山羊血液DNA样本鉴定结果与实际性别对比,雌性准确率为100%（22/22）,雄性准确率为100%（24/24）。

英文摘要：

Based on the characteristic of the hircine AMEL-X and AMEL-Y gene with various degree deletions,a pair of primers were utilized to establish the process of sex identification by PCR and agarose gel electrophoresis with mixed samples of the hircine blood genomic DNA,and the PCR produced was purified,cloned and sequenced,then sex identification to the 46 hircine blood samples（♀：24,♂：22）.The results showed that the male hircine sample produces a non-specificity band and a specificity band,while the female hircine sample produces one band.The non-specificity fragment 349 bp,the specificity fragment 289 bp.By comparison,the results of the 46 hircine blood DNA samples sex identification with the hircine actual sex,the female accuracy 100%（22/22）,the male accuracy 100%（24/24）.