中文摘要：

目的 建立体外培养人肺内微小动脉的方法 ,并观察其对收缩剂反应的基本特性,为研究药物及各种活性因子对人肺血管的长期作用提供有效的实验模型。方法 取癌旁5 cm以上的肺组织,于冰浴和无菌条件下,利用显微操作分离出肺内微小动脉,制备成1.8-2.0 mm长的血管条,分两组,一组急性分离组（新鲜组）马上进行血管张力测定;另一组（培养组）置于含DMEM-F12培养基（内含10%胎牛血清、1%青链霉素）的培养皿内,通以95%O2、5%CO2混合气,在37℃的条件下培养48 h,再进行张力测试。进行血管张力测定时,用两根直径40μm的不锈钢丝平行穿过血管腔,钢丝的四个端分别固定于微血管张力测定仪浴槽内钳夹的四个螺丝上。采用累积给药法,分别给予血管受体依赖性收缩剂血栓素A2类似物（U46619）和内皮素-1（endothelin-1,ET-1）,血管非受体依赖性收缩剂60 mmol·L-1 KCL,然后测定血管的张力变化。结果 培养组对血管收缩剂U46619和ET-1能产生浓度依赖性收缩,U46619的p D2为7.60±0.10,Emax为136.40%±6.17%（n=11）;ET-1的p D2为7.17±0.22,Emax为137.14%±5.52%（n=9）,结果 类似于新鲜组：U46619的p D2为7.78±0.11,Emax为131.29%±3.79%（n=13）;ET-1的p D2为7.53±0.15,Emax为139.11%±6.66%（n=10）,两组比较,差异均无统计学意义（P〉0.05）。培养组对血管非受体依赖性收缩剂60mmol·L-1 KCL产生的收缩力Emax为（7.67±0.85）m N（n=12）;新鲜组的Emax为（7.73±0.97）m N（n=8）,两组比较,差异无统计学意义。结论 采用血管器官培养的方法 培养人肺内微小动脉48 h,能维持血管平滑肌的基本收缩特性,可作为研究各种物质对血管长效作用的有效模型。

英文摘要：

Objectives To establish organ culture technique of human intrapulmonary artery and investigate the basic contractile characteristics of organ-cultured arteries, providing a useful model for studying the long-term effects of drugs and various growth factors on human pulmonary arteries. Methods The apparently normal human lung tissue, more than 5 cm distant from the lung cancer tissue, was excised from the lung lobe at surgery. Then the intrapulmonary artery was isolated with micromanipulation in sterile, ice-cold Krebs solution. After removal of fat and connective tissue, the intrapulmonary artery was cut into several ring segments （1.8-2.0 mm in length）. They were divided into two groups,the acute isolation （fresh） group and the organ-cultured group. The segments from the organ-cuhured group were incubated in Dulbecco＇s Modified Eagle Medium-F12 with 10% fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin, at 37℃ with humidified 95% 02 and 5% CO2 for 48 h. While vascular tension was determined, two tungsten wires （each 40μm in diameter） were inserted through the segment＇s lumen, and each wire was fixed to the four jaws of a myograph. With a cumulative addition of drug, effects of the receptor-dependent vasoconstrictors [ （U46619 and endothelin- 1 （ET- 1 ） ] and receptor-independent vasoconstrictor （60 mmol. L-1 KCL） on intrapulmonary arterial tings were determined. Results In organ-cultured group, U46619 （pD2： 7.60＋0.10, Emax： 136.40%±6.17%, n= 11 ） and ET- 1 （pD2 ： 7.17＋0.22, Emax： 137.14%±5.52%, n=9） could produce a concentration-dependent contraction response. The results were comparable with the fresh group, which were U46619 （pD2： 7.78±0.11, Emax： 131.29%＋ 3.79%, n=13） and ET-1 （pD2： 7.53＋0.15, Emax： 139.11%±6.66%, n=10） respectively. The receptor-independent vasoconstrictor KCL （ 60 mmol. L-1 ） -induced contractions [ tension ： （ 7.67 ±0.85 ）mN in organ -cultured group, n = 12 and （7.73±0.97）mN in fresh