中文摘要：

目的检测地榆皂苷II对多种肿瘤细胞的体外增殖抑制作用,研究地榆皂苷II诱导肿瘤细胞凋亡的分子机制。方法采用SRB法检测地榆皂苷II对肿瘤细胞的体外增殖抑制,通过Western blot来研究地榆皂苷II对三大凋亡通路的影响。结果地榆皂苷Ⅱ可上调MDA-MB-231细胞中活性氧的水平,上调CHOP的表达,激活内质网应激途径;下调Bcl-2的表达,上调Bax的表达,激活线粒体凋亡途径;上调DR4、DR5的表达,激活TRAIL外源凋亡通路。结论地榆皂苷II通过激活三大细胞凋亡通路来抑制肿瘤细胞的增殖,促进肿瘤细胞的凋亡。

英文摘要：

OBJECTIVE To study the inhibition effects of ziyuglycoside Ⅱ on various tumor cells and the molecular mechanism underlying the ziyuglycosideⅡ-induced apoptosis of tumor cells. METHODS SRB method was used to measure the effect of ziyuglycosideⅡon the proliferation of cancer cells in vitro and western blot was used to test influences of ziyuglycosideⅡ on three pathways of apoptosis. RESULTS The intracellular ROS level of MDA-MB-231 was significantly up-regulated when treated with ziyuglycosideⅡ,which resulted the expression of CHOP and triggered the endoplasmic reticulum stress response. Besides,we observed that the expression of Bcl-2 was downregulated while the expression of Bax was up-regulated,which suggested the activation of mitochondrial apoptosis pathway. We also noticed the up-regulation of DR4 and DR5,which associated with the activation of death receptor pathways. CONCLUSION Through the activation of three apoptosis pathways,ziyuglycoside Ⅱ can inhibit the proliferation and promote the apoptosis of tumor cells.