中文摘要：

以豌豆根瘤菌（Rhizobium leguminosarum 11723）为出发菌株，利用紫外-LiCl和超声波进行诱变，以期获得高产辅酶Q10菌株。结果表明：lg／LLiCl诱变能显著提高菌株的正突变率，超声波诱变会引起细胞壁结构与构成发生改变。通过罗红霉素初筛和摇床复筛，获得辅酶Q10突变株C40-05，胞内辅酶QlO产量为1．198mg／g干菌，比出发菌株（0．389mg／g）提高了208％。菌株经5次传代培养，胞内辅酶Q10产量下降了2．67％，突变株遗传形状稳定，可作为辅酶Q10生产菌株。

英文摘要：

Rhizobium leguminosarum 1.1723 was mutagenized by means of LiCl treatment followed by UV irradiation or ultrasonic treatment alone to yield coenzyme Q10-producing strains. The results showed that 1 g/L LiCl could obviously increase the positive mutation rate and that ultrasonic treatment could change cell wall structure of Rhizobium leguminosarum. A mutant C40-05 with high productivity of coenzyme Q10 was obtained through preliminary screening with roxithromycin and secondary screening by means of shake flask cultivation. Its coenzyme Q10 yield was up to 1.198 mg/g, which revealed a 208% increase compared with the original stain. Only 2.67% reduction in coenzyme Q10 yield of the mutant was observed after the fifth passage. Therefore, the obtained mutant is a stable strain that deserves to be studied further.