中文摘要：

应用PCR技术,直接从土壤中抽提总DNA,扩增16S rDNAV3片段,采用变性梯度凝胶电泳（DGGE）分析16S rDNAV3片段的多态性,研究了杭州西湖梅家坞不同植茶年龄（8、50和90年）、不同利用方式（茶园、荒地和林地）的土壤微生物群落基因多样性.结果表明：不同植茶年龄和不同土地利用方式影响土壤微生物群落的基因多样性.荒地、茶园和林地土壤微生物群落基因多样性指数明显不同（P〈0.05）,其排列顺序为荒地〉茶园〉林地.不同植茶年龄的土壤中,50年茶园土壤的微生物群落基因多样性指数、微生物量碳和基础呼吸明显高于8年和90年茶园土壤（P〈0.05）.

英文摘要：

In this paper, the total microbial DNA was extracted from the soils in 8-, 50- and 90 years old tea orchards, adjacent wasteland, and 90 years old forestland in Meijiawu tea area of Hangzhou. The 16S rDNA V3 fragment was amplified by PCR, and the polymorphism of this fragment was analyzed by DGGE. The results indicated that both the tea orchard age and the land use type had significant effects on soil microbial genetic diversity. There was a significant difference （P 〈 0. 05 ） in the microbial genetic diversity index among wasteland, tea orchards and forestland, which was decreased in the order of wasteland 〉 tea orchard 〉 forestland. For the tea orchards of different ages, the soil microbial genetic diversity index, significantly higher in 50 years old than in 8 and microbial biomass C, and basal respiration were 90 years old tea orchards.