中文摘要：

目的：通过测定胃窦平滑肌细胞内信使物质钙离子浓度及三磷酸肌醇、环磷酸腺苷含量的变化，探讨针刺足阳明经穴对胃窭平滑肌细胞内信息转导通路的影响。 方法：实验于2003-06／2004-05在湖南中医学院针灸基础实验室完成。选用清洁级健康成年新西兰大耳白兔55只，随机分为5组，每组11只。①生理盐水组耳缘静脉滴注9g／L氯化钠溶液。②阿托品组耳缘静。脉滴注0．25mg／L阿托品。③电针足阳明经穴组、电针足少阳经穴组和电针足太阳经穴组均在滴注阿托品总量的一半时，分别电针双侧足阳明经穴、足少阳经穴、足太阳经穴30min。各组分别处理后，分离胃窦平滑肌细胞，制成活的单个平滑肌细胞悬液；测定胞内钙离子浓度采用荧光法；测定胞内三磷酸肌醇含量应用蛋白质竞争结合分析法；测定胞内环磷酸腺苷含量采用放射免疫计数法。 结果：纳入家兔55只，每组11只。最终进入结果分析动物数为：钙离子浓度分析55只，每组11只。无脱失；三磷酸肌醇及环磷酸腺苷含量分析数均为45只，均脱失10只。①各组家兔胃窦平滑肌细胞内钙离子浓度的比较：阿托品组、足少阳经穴组与足太阳经穴组钙离子浓度明显低于足阳明经穴组[（172．97±60．38），（203．57±72．20），（183．52±76．81），（321．22±55．36）nmol／L（F=9．37，P〈0．01）]。②各组家兔胃窦平滑肌细胞内三磷酸肌醇含量的比较：阿托品组、足少阳经穴组、足太阳经穴组三磷酸肌醇含量均明显低于足阳明经穴组[（21．87±10．16），（21．08±10．97）。（23．81±12．06）。（39．00±12．97）pmol／10^6 F=3．61，P〈0．01）]。③各组家兔胃窭平滑肌细胞内环磷酸腺苷含量的比较：各组差异均无显著性意义（P〉0．05）。 结论：针刺足阳明经穴可使家兔胃窦平滑肌细胞内钙离子、三磷酸肌醇古?

英文摘要：

AIM： To probe into the effect of electro-acupuncturing aeupoints of the Foot-Yangming channel on signal transduction pathway in gastric sinus smooth muscle cell through detecting the changes of contents of intracellular Ca^2＋, inositol triphosphate （IP3） and cyclic adenosine monophosphate （cAMP）, METHODS： The experiment was conducted at the laboratory of acupuncture, Hunan College of Traditional Chinese Medicine from June 2003 to May 2004. Fifty-five clean-grade healthy adult New Zealand flap-eared white rabbits were selected and assigned randomly into 5 groups with 11 rabbits in each group.（1）The saline control group： instilling 9 g/L NaCl solution into ear edge vein; （2）Atropine group： instilling 0.25 mg/L atropine into ear edge; （3）Foot-Yangming channel acupoints group, the FootShaoyang channel acupoints group and the Foot-Taiyartg channel acupoints group： After instilling half amount of atropine into ear edge, electroacupuncture was performed at Foot-Yangming channel acupoints, Foot-Shaoyartg channel acupoints and Foot-Ta/yang channel acupoints, respectively for 30 minutes. Having been treated, individual cells were isolated from gastric antrum circular smooth muscle cells. Alive suspending cell liquid was prepared. The concentration nf intracellular Ca^2＋ was detected by fluorescing method. The levels of IP3 and cAMP were detected with Competitive Protein Binding Assay （CPBA） and radioimmunoassay counting method, respectively. RESULTS： Fifty-five rabbits were involved, 11 rabbits in each group. Final number of including into result analysis： 55 rabbits in the analysis of concentration of Ca^2＋, 11 in each group, no drop out; 45 rabbits in the analysis of contents of IP3 and camp, and all had 10 drop out, （1）Comparison of concentration of Ca^2＋ in gastric antrum circular smooth muscle cells of every group： It was lower significantly in the atropine group, Foot-Shaoyang channel acupoints group and Foot-Taiyang channel acupoints group than that in the Foot