中文摘要：

目的探讨发酵虫草菌粉对糖尿病心肌病的治疗作用及其对心肌核苷酸结合寡聚化结构域样受体3（NLRP3）、半胱氨酸天冬氨酸蛋白酶1（Caspase-1）、白细胞介素-1β（IL-1β）表达的影响。方法雄性SD大鼠45只，随机分为正常对照组（CON组）、糖尿病组（DM组）和发酵虫草菌粉治疗组（CS组，1g·kg-1·d-1），每组15只。DM组和CS组大鼠通过尾静脉注射链脲佐菌素（45mg/kg）诱导糖尿病模型。CS组每日给予1次发酵虫草菌粉溶液灌胃，CON组和DM组给予相同体积生理盐水灌胃。灌胃18周后取材。HE染色观察心肌组织形态学改变；电镜观察心肌超微结构改变；Westernblotting法检测心肌NLRP3的表达；免疫组化法检测各组大鼠心肌NLRP3、Caspase-1、IL-1β的表达水平。结果（1）电镜下，DM组大鼠出现局部心肌纤维变性坏死、部分线粒体损伤；CS组大鼠心肌病变较DM组明显减轻。（2）Westernblotting结果显示，DM组和CS组大鼠心肌NLRP3表达均较CON组显著上升，CS组大鼠心肌NLRP3表达较DM组降低。（3）免疫组化结果显示，DM组和CS组大鼠心肌NLRP3、Caspase-1、IL-1β表达均较CON组显著上升，CS组均较DM组降低。结论发酵虫草菌粉能减轻糖尿病大鼠心肌病变，该作用可能与其抑制心肌NLRP3、Caspase-1、IL-1β的表达有关。

英文摘要：

Objective To investigate the therapy effect of fermented cordyceps powder on diabetic cardiomyopathy,and the effect on expressions of NLRP3,Caspase-1and interleukin(IL)-1βin myocardium of diabetic rats.Methods Atotal of45male SD rats were randomly divided into normal control group(group CON),diabetes group(group DM)andfermented cordyceps powder administration group(group CS,1g·kg-1·d-1).Diabetes rat model was induced by tail veininjection with streptozotocin(45mg/kg)in group DM and group CS.The rats in group CS were given fermented cordycepspowder solution by gavage at a single dose of1g·kg-1·d-1for18weeks.Rats of group CON and group DM were given0.9%NaCl in the same way.All rats were sacrificed after18weeks.The pathological changes in myocardium were observed by HEstaining and electron microscope.The expression of NLRP3in myocardium was detected by Western blotting.Theexpression levels of NLRP3,Caspase-1and IL-1βin myocardium were detected by immunohistochemistry.Results(1)Myocardial necrosis and fibrosis and mitochondrial damages were observed under electron microscope in group DM.Theabove-mentioned pathological changes were improved in the group CS.(2)The result of Western blotting showed that theexpression of NLRP3in myocardium was significantly increased in group DM and group CS than that in group CON,and theexpression of NLRP3was significantly lower in group CS than that in group DM.(3)The result of immunohistochemistryshowed that,compared with the group CON,the expressions of NLRP3,Caspase-1and IL-1βin myocardium weresignificantly increased in group DM and group CS,and which were significantly lower in group CS than those in group DM.Conclusion Fermented cordyceps powder can improve diabetic cardiomyopathy through decreasing the expressions ofNLRP3,Caspase-1and IL-1βin myocardium.