中文摘要：

目的研究脂多糖（lipopolysaccharide，LPS）致小鼠急性肺损伤（acute lung injury，ALI）时β-连环素（-βcatenin，-βcat）在气道上皮细胞的表达变化及其意义。方法在建立LPS气管注射致小鼠急性肺损伤模型基础上，应用免疫组织化学染色检测-βcat、糖原合成酶激酶3-β（GSK3-β）在气道上皮的表达；应用Western blot检测肺组织内蛋白激酶C（PKC）、GSK3-β、磷酸化GSK3-β（P-GSK3-β）以及-βcat蛋白的表达。结果免疫检测显示，与正常组小鼠相比，LPS致急性肺损伤的小鼠气道上皮中，胞膜上-βcat及胞质内GSK3-β蛋白含量均明显降低（均P〈0.05）；Western blot检测显示，肺组织内PKC、P-GSK3-β及-βcat蛋白含量均较正常组小鼠明显升高（均P〈0.05），而GSK3-β蛋白含量显著下降（P〈0.05）。结论由LPS所致的小鼠ALI，一方面引起胞膜上行使连接功能的-βcat含量显著下降；另一方面可能通过PKC激活、GSK3-β磷酸化失活，导致胞质内-βcat含量的增加，使-βcat转入核内并与转录因子结合，启动Wnt途径靶基因转录，从而在气道上皮的损伤修复中发挥重要作用。

英文摘要：

Objective To investigate the expression of β-catenin （β-cat） in the airway epithelial cells of acute lung injury induced by LPS and its significance. Methods The mouse model of acute lung injury （ALI） was established by intrathacheal injection of LPS. The SP immunohistochemical method was used to detect the expression of β-cat, glycogen synthase kinase3-β （GSK3-β） in the airway epithelial cells. Western blot was used to detect the expression of protein kinase C （PKC）, GSK3-β, phosphoric GSK3-β （P-GSK3-β） and β-cat proteins in lung tissues. Results The results of SP immunohistochemical method showed that the expression of β-cat on the airway epithelial membrane and that of GSK3-β in the cytoplasm were remarkably decreased in model group than in control group （both P〈0.05）. The results of Western blot revealed that the expression of PKC, P-GSK3-β and β-cat proteins in lung tissues was significantly increased （all P〈0.05）, but that of GSK3-β protein was significantly decreased in ALI group as compared with that in control group （P〈0.05）. Conclusion The expression of β-cat on the airway epithelial membrane was decreased, but that in the cytoplasm was increased by the activation of PKC and the inactivation of GSK3-β phosphorylation in the mice with LPS-induced ALI. β-cat was shifted into the nucleus and bond with transcriptional factor to initiate the transcription of the target genes of Wnt pathway, so played an important role in the repair of the airway epithelial injury.