中文摘要：

目的探讨次要组织相容性抗原13（ minor histocompatibility antigen 13, HM13 ）蛋白在胃癌组织中的表达情况及其与患者临床病理指标和预后的关系。方法应用免疫组化法检测90例胃癌及癌旁组织中HM13蛋白的表达，并分析其与胃癌患者临床病理指标及预后的相关性：qRT—PCR检测HM13干扰后下游基因血红素氧化酶1的表达改变。结果胃癌组织中HMl3的表达比癌旁正常组织中的表达降低，二者的高表达率分别为47％和61％，但差异无统计学意义（疋。=3．78，P=0．052）。HM13蛋白表达与胃癌TNM分期（X2＋=5．022，P=0．025）及远处转移（P=0．033）有关，而与患者年龄（X^2=0．832，P=0．362）、性别（X^2=0．779，P=0．378）、肿瘤大小（X^2=0．804，P=0．370）、分化程度（X^2=0．430，P=0．512）、大体类型（X^2=2．069，P=0．150）、浸润深度（X^2=0．167，P=0．683）及有无淋巴转移（X^2=0．396，P=0．529）无关。HM13蛋白低表达的胃癌患者比高表达胃癌患者的预后差（中位总生存期：15个月比54个月，X^2=6．456，P=0．0u；中位无进展生存期：13个月比48个月，X^2=6．742，P：0．009）。干扰HM13表达后血红素氧化酶1表达上调（0．532±0．013比0．395±0．011．t=13．93，P〈0．05）。结论HM13低表达与肿瘤的进展、转移及不良预后密切相关。

英文摘要：

Objective To explore the expression of minor histocompatibility antigen 13 （HM13） in gastric carcinoma and the relationship with clinicopathological parameters and prognosis. Methods The expression of HM13 was detected by immunohistochemistry in a total of 90 pairs of paraffin-embedded gastric cancer tissue specimens and corresponding paraneoplastic tissues. The correlation between clinicopathologieal parameters and the expression of HM13 in gastric carcinoma were also analyzed. Downstream gene heme oxygenase-1 （ HO-1 ） expression was detected by qRT-PCR after HM13 gene was interfered. Results High expression of HM13 protein was observed in 47% gastric carcinoma compared with that in 61% corresponding paraneoplastic tissues （X2 = 3.78, P = 0. 052）. HM13 expression has positive correlation with pathological TNM stage （X2 = 5.022, P = 0. 025 ） and distant metastasis （ P = 0. 033）, but not with age （X2 = 0. 832, P = 0. 362）, gender （X2 = 0. 779, P = 0. 378 ）, tumor size （X2 = 0. 804, P = 0. 370 ）, tumor differentiation （X2= 0.430, P = 0. 512 ）, gross types （Xz = 2. 069, P = 0. 150）, tumor stromal invasion （X2 = 0. 167, P = 0.683） and lymph node status （X2 = 0.396, P = 0. 529 ）. The patients with low HMI3 expression had worse overall survival [ （OS） ： （30±5 ） months vs. （47±5） months, Xz =6. 456, P =0. 011 ] and progress free survival [ （PFS） ： （29±5） months vs. （46±5 ） months, X2 = 6. 742, P = 0. 009 ]. Expression of HO-1 was up-regulated after HM13 gene was interfered （0.532±0.013 vs. 0.395±0.011, t=13.93, P〈0.05）. Conclusion The low expression of HM13 is assoeiated closely with advanced stage and distant metastases of gastric carcinoma.