中文摘要：

目的 研究mieroRNA-217（简称miR-217）在结直肠癌中的表达及临床意义.方法 收集30例结直肠癌患者肿瘤原发灶和相应癌旁正常黏膜组织标本,应用实时荧光定量聚合酶链式反应（PCR）方法检测miR-217的表达,独立样本均数采用t检验分析miR-217的表达差异,Fisher精确检验分析其与相关临床病理指标的关系,Kaplan-Meier法分析其与预后的关系.结果 与相应的正常结直肠黏膜组织相比,30例结直肠癌组织中miR-217显著低表达（3.3±0.6比5.3±0.7,P=0.029 ＜0.05）,肿瘤直径＞2 cm的（17例）患者的miR-217表达明显低于肿瘤＜2 cm（13例）患者（P =0.025）;TNM分期为Ⅲ～Ⅳ期的（15例）患者的miR-217的表达水平明显低于Ⅰ～Ⅱ期的（15例）患者,差异有统计学意义（P=0.025）;有周围淋巴结转移和远处转移的患者的miR-217的表达显著低于无淋巴结转移和远处转移的患者,差异具有统计学意义（P =0.033,P=0.026）.miR-217低表达结直肠癌患者（17例）平均生存时间明显短于高表达患者（13例）[（12.0±3.3）比（33.0±7.6）个月],差异有统计学意义（χ^2 =4.584,P=0.032）.结论 miR-217在结直肠癌中显著低表达,其可能作为一种标志物预测结直肠癌的恶性程度.

英文摘要：

Objective To investigate microRNA-217 expression and its significance in colorectal cancer.Methods MiR-217 expression was detected in 30 cases of colorectal cancer tissues and corresponding adjacent normal colorectal tissues by qRT-PCR.The clinicopathologic correlations of miR-217 expression were performed using Fisher＆#39;s exact test.Kaplan-Meier method was used to evaluate the overall survival of patients.Results MiR-217 was significantly lower in colorectal cancer tissues than in normal colorectal tissues （P 〈 0.01）.Moreover,the decreased miR-217 level was significantly associated with tumor size and TNM stage,as well as lymph node and distant metastasis.Meanwhile,patients of colorectal cancer with low levels of miR-217 expression had a significantly shorter median survival （χ^2 =4.584,P 〈 0.05） than those with high levels of miR-217 expression.Conclusions Downregulated miR-217 in colorectal cancer tissues correlates to poor prognosis in postoperative colorectal cancer patients.