中文摘要：

背景胰腺的癌症的高死亡率与长期的功效为进一步的治疗成为一个瓶颈。发现一个新平均数精确地预言胰腺的癌症的早发作是迫切的。作者假设了那在朊酶的反常激活的胰岛素表示 / 功能和结果激活的基因能影响的 cationic trypsinogen (PRSS1 ) 的基因变体 receptor-2 (PAR-2 ) ，然后导致胰腺的癌症。这研究的目的是完全与胰腺的 cancer.Methods 在病人详细描述 PRSS1 基因的一些新奇变化有胰腺的癌症和控制在这被注册的 220 个无关的个人的 156 个病人学习。PRSS1 基因的变化被直接定序分析。K 地岬变化察觉工具包被用来在胰腺的癌症组织发现一般 k 地岬基因混乱。然后，临床的数据在那里被收集并且分析 simultaneously.Results 是带了是在外部血标本和胰腺的癌症织物的 PRSS1 基因的 IVS 3 +157 GC 的新奇变化的二个病人。而且，它令人吃惊在另一个年轻病人在 PRSS1 基因(c.409 AG 和 c.416 CT ) 发现 exon 的一个新奇复杂变化 3。成为号码 135 的复杂变化和 No.137 氨基酸从 Thr 转到翼和 Thr 到分别地相遇了。当 k 地岬基因的变化都没在 PRSS1 基因的三个 patients.Conclusion 变化被检测时，没有任何变化在正常控制被发现可以是胰腺的癌症的一个重要因素。

英文摘要：

Background A high mortality rate of pancreatic cancer becomes a bottleneck for further treatment with long-term efficacy. It is urgent to find a new mean to predict the early onset of pancreatic cancer accurately. The authors hypothesized that genetic variants of cationic trypsinogen （PRSS1） gene could affect trypsin expression/function and result in abnormal activation of protease activated receptor-2 （PAR-2）, then lead to pancreatic cancer. The aim of this study was to elaborate some novel mutations of PRSS1 gene in the patients with pancreatic cancer. Methods Totally 156 patients with pancreatic cancer and 220 unrelated individuals as controls were enrolled in this study. The mutations of PRSS1 gene were analyzed by direct sequencing. K-ras Mutation Detection Kit was used to find the general k-ras gene disorder in the pancreatic cancer tissue. Then the clinical data were collected and analyzed simultaneously. Results There were two patients who carried novel mutations which was IVS 3 ＋157 G〉C of PFISSI gene in peripheral blood specimens and pancreatic cancer tissue. What＇s more, it was surprising to find a novel complicated mutation of exon 3 in PRSS1 gene （c.409 A〉G and c.416 C〉T） in another young patient. The complicated mutation made No.135 and No.137 amino acid transfer from Thr to Ala and Thr to Met respectively. No any mutation was found in the normal controls while no mutations of k-ras gene were detected in the three patients. Conclusion Mutations of PRSS1 gene may be an important factor of pancreatic cancer.