中文摘要：

采用还原法制备了AuNPs／MWCNTs复合材料，并构建了氧化还原蛋白质的固定化和生物传感界面AuNPs／MWCNTs／GC电极．以肌红蛋白（Myoglobin，Mb）为例，研究了固定化蛋白质在AuNPs／MWCNTs／GC电极上的直接电化学．结果表明，AuNPs／MWCNTs复合材料不仅能有效地促进Mb与电极表面的直接电子转移，而且能很好地保持固定化Mb的生物催化活性．Mb／AuNPs／MWCNTs／GC电极对H，0，具有良好的电催化还原性能，其线性响应范围为1～138μmol·L^-1，检测限为0．32μmol·L^-1（S／N=3），并具有较低的米氏常数（0．143mmol·L^-1）．该电极操作简单，响应迅速，稳定性和重现性好，有望用于蛋白质的固定化及第三代生物传感器的制备．

英文摘要：

A novel matrix, multiwalled carbon nanotubes supported Au nanoparticles composite nanomaterial （AuNPs/MWCNTs） for immobilization of protein and biosensing was designed using a simple and effective one-step in situ synthesis route. Using myoglobin （Mb） as a model, the direct electrochemistry of the immo- bilized proteins on the AuNPs/MWCNTs composite was studied. The results showed that the AuNPs/MWC- NTs composite can maintain the bioactivity and facilitate the direct electrochemistry of Mb in the Mb/AuNPs/ MWCNTs/GC electrode. Based on the direct electron transfer of the immobilized Mb, the protein electrode exhibited excellent electrocatalytic activity to the reduction of H202 with a linear range from 1 - 138 μmol·L^-1, low detection limit of 0.32 μmol·L^-1（ S/N = 3） and a low apparent Kapp value of 0. 143 mmol·L^-1. The simple operation, fast response and well reproducibility of the proposed biosensor indicated its promising appli-cations in protein immobilization and preparation of the third generation biosensors.