中文摘要：

目的探讨系统性红斑狼疮（systemic lupus erythematosus，SLE）患者CD4^＋、CD8^＋T淋巴细胞SLAM基因的表达。方法采用免疫磁珠的方法对20例系统性红斑狼疮患者及10例正常人外周血CD4^＋、CD8^＋T淋巴细胞进行分离，RT—PCR对CD4^＋、CD8^＋T淋巴细胞SLAM基因表达进行分析。结果SLE患者CD4^＋、CD8^＋T淋巴细胞SLAM mRNA表达增加显著高于正常对照。结论SLAM介导的T淋巴信号通路转导异常可能在SLE患者T淋巴细胞激活异常中发挥重要的作用。

英文摘要：

Objective To explore the expression of SLAM gene in the CD4^＋ and CD8^＋ T cells from SLE patients. Method The CD4^＋ and CD8^＋ T cells from peripheral blood of 20 SLE patients and 10 healthy blood donors were separated using magnetic cell sorting system （MACS）. We detected the expression of SLAM mRNA in CD4^＋ and CD8^＋ T cells by RT-PCR. Results The CD4^＋ and CD8^＋ T cells from SLE patients showed significantly higher SLAM expression than those from healthy blood donors. Conclusion The SLAM signal pathway may play an important role in contributing to the abnormal activation of T cells in SLE patients.