中文摘要：

目的 探讨抗凋亡基因Hax—1在系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中的表达水平及其在SLE发病中的作用及意义。方法 运用GLGI技术对SLE患者CD4CF细胞LongSAGE文库中单一匹配标签T4Y7进行鉴定和序列分析，进一步应用反转录一聚合酶链反应（RT—PCR）检测34例活动期SLE患者和25名正常人PBMC中Hax—1 mRNA的表达水平。结果 活动期SLE组Hax—1 mRNA的表达水平明显高于正常对照组，两者间差异有统计学意义（Z=-4．556，P＜0．01）；中度及重度活动期SLE患者PBMC中Hax—1 mRNA表达水平显著高于轻度活动组，两者间差异均具有统计学意义（P＜0．01）。结论 活动期SLE患者PBMC中Hax—1 mRNA的表达较正常人明显增加，且与SLE的疾病活动性呈一定的线性相关关系。

英文摘要：

Objective To study the mRNA expression of HS1-associated protein X-1 （Hax-1）, an an-ti-apoptosis gene, in the peripheral blood mononuclear cells （PBMC） of patients with systemic lupus erythe matosus （SLE）, and further investigate the roles and significance of Hax-1 in the pathogenesis of SLE. Methods Generation of longer cDNA fragments from serial analysis of gene expression （SAGE） tags for gene identification （GLGI） was applied to identify the gene Hax-1 according to the Long SAGE tag. Then reverse transcription-polymerase chain reaction （RT-PCR） technique was used to semiquantitatively analyze mRNA expressions of Hax-1 inPBMC from 34 active SLE patients and 25 healthy subjects. Results Compared with healthy controls, there was significant difference between SLE patients in the active stage and the normal controls （Z=-4.556, P〈0.01）. The average level of mRNA expression in active SLE group was higher than that in healthy controlS. Significant difference was found between the group with mild SLE and either the moderate or the severe one （P〈0.01）. Conclusion The mRNA expression level of Hax-1 in active SLE group increase markedly, and to some extent, it is related to the activity of SLE. This provides a valuable basis for the further study on the role of apoptosis in SLE.