中文摘要：

为了在大肠杆菌中分段表达人类TES基因C端3个顺次排列的LIM结构域，利用PCR技术从含有TES基因全长编码序列的质粒pCMV-HA-TES中扩增出这3个LIM domain基因片段，分别重组入pGEM-T载体，再经酶切后，将获得的目的片段亚克隆入原核表达载体pQE-N1进行融合表达。酶切鉴定及测序结果表明，扩增产物与已知基因序列一致,成功构建了3个目的片段的pQE-N1表达质粒；SDS-PAGE结果显示，IPTG诱导表达出与预期分子质量大小相同的3个融合蛋白。

英文摘要：

In order to express three tandem LIM domains in the C-terminus of TES individually in Escherichia coli BL 21, three gene fragments encoding corresponding LIM domains in TES C-terminus were amplified from pCMV-HA-TES plasmid containing the full length of TES coding sequence by PCR and cloned into pGEM-T vector respectively. The target gene fragments obtained by digesting the recombinant plasmids with restriction endonuclease were subcloned into prokaryotic expression vector pQE-N1 for fusion expression. The results of restriction endonuclease digestion and sequence analysis indicated that three recombinant plasmids for expression of corresponding LIM domains in TES C-terminus were successfully constructed. The fusion proteins with the molecular weights as prospected were separated by SDS-PAGE gel after induced expression.