中文摘要：

DNA 免疫是为高亲密关系的 monoclonal 抗体产生的一个有效方法。这里，我们对松香油绑定蛋白质 4 描述几高质量的 monoclonal 抗体(mAbs ) 的产生(RBP4 ) ，为肾反常和机能障碍的一个重要标记，与 DNA 的一个联合方法， priming 和蛋白质增加。产生的 mAbs 能与高敏感并且用这些 mAbs 绑在 RBP4，一块 immunocolloidal 金牌快测试长带被构造。长带能交上结果 < 5 min 并且与大约 1 ng/ml 的察觉限制是很敏感的。小规模的临床的测试表明这脱衣的结果根据在市场上当前可得到的一个标记酶的 immunosorbent 试金工具包的好。因而，它能为在诊所的更方便、更快的 RBP4 决心是有用的。

英文摘要：

DNA immunization is an efficient method for high-affinity monoclonal antibody generation. Here, we describe the generation of several high-quality monoclonal antibodies （mAbs） against retinol-binding protein 4 （RBP4）, an important marker for kidney abnormality and dysfunction, with a combination method of DNA priming and protein boost. The mAbs generated could bind to RBP4 with high sensitivity and using these mAbs, an immunocoHoidal gold fast test strip was constructed. The strip can give a result in 〈5 rain and is very sensitive with a detection limit of about 1 ng/ml. A small-scale clinical test revealed that the result of this strip was well in accordance with that of an enzymelabeled immunosorbent assay kit currently available on the market. Consequently, it could be useful for more convenient and faster RBP4 determination in the clinic.