中文摘要：

Cenp-F (也命名的 mitosin ) 是为有丝分裂的前进重要的 350-kDa 人 kinetochore 蛋白质。它也是在分裂期间房间的原子矩阵蛋白质。这里，我们显示出进变化的众多的总数的染色质冷凝作用在分裂期间原子核缩放的包含 112 残余导致了的 C 终端的 Cenp-F 的 N 终端删除异种的那 overexpression，有外长的蛋白质的 colocalizing。在用整个染色体油漆的 situ 杂交，探针显示染色质总数不是过早地压缩的单个染色体。任何一个都不是他们由于 apoptosis。我们提供了与分裂期间染色质纤维的某些区域显示出 Cenp-F 的协会的证据。与 DNA 依赖的蛋白质 kinase (DNA-PK ) 联系的 Cenp-F，为染色体动态平衡批评的 trimeric 蛋白质建筑群。而且， Cenp-F 异种的 DNA-PK 协会活动与他们导致染色质聚集的能力相关。这些结果通过协会并且可能在分裂期间染色质的组织暗示 Cenp-F 的一个角色 DNA-PK 的规定。

英文摘要：

Cenp-F （also named mitosin） is a 350-kDa human kinetochore protein important for the mitotic progression. It is also a nuclear matrix protein in interphase cells. Here, we showed that overexpression of N-terminal deletion mutants of Cenp-F containing the C-terminal 112 residues induced chromatin condensation into numerous aggregates of varying sizes in interphase nucleus, colocalizing with the exogenous proteins. In situ hybridization using whole chromosome painting probes indicated that the chromatin aggregates were not prematurely condensed individual chromosomes. Neither were they due to apoptosis. We provided evidence showing association of Cenp- F with certain regions of interphase chromatin fibers. Cenp-F associated with the DNA-dependent protein kinase （DNA-PK）, a trimeric protein complex critical for genome homeostasis. Moreover, the DNA-PK association activity of Cenp-F mutants correlated with their ability to induce chromatin aggregation. These results imply a role of Cenp-F in organization of interphase chromatin through association and possibly regulation of DNA-PK.