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海洋卡盾藻(香港株)溶血活性特征研究

期刊名称：环境科学
时间：0
页码：2920-2925
语言：中文
分类：X171.5[环境科学与工程—环境科学]
作者机构：[1]暨南大学赤潮与水环境研究中心,广州510632, [2]华南师范大学生命科学学院,广州510630
相关基金：国家自然科学基金重点项目（U0733006）; 国家重点基础研究发展规划（973）项目（2010CB428702）; 广东908赤潮灾害调查专项（GD908-01-07）; 广东908赤潮灾害评价专项（GD908-02-14）; 海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室开放基金项目（MATHAB200909）
相关项目：广东沿海鱼毒性赤潮发生的营养特征与致毒机制研究

关键词：海洋卡盾藻, 溶血毒素, 溶血活性, 卤虫, 营养盐限制, 急性毒性试验, Chattonella marina Hong Kong strain, hemolytic toxins, hemolytic activity, Artemia sinica, nutrients limitation, acute toxicity test

中文摘要：

研究了海洋卡盾藻香港株（Chattonella marina,Hong Kong strain,CMHK）在不同生长阶段和营养条件下的溶血活性特征.根据海洋卡盾藻的生长曲线,分别收集对数生长期、稳定期、衰亡期的藻细胞进行溶血毒性的测定;采用不同营养元素结构的培养基对CMHK进行培养,对不同培养条件下的藻细胞进行溶血活性的测定;采用对数生长初期（5 d）、对数期末期（13 d）、稳定期（15 d）和衰亡期（17 d）的CMHK藻液对卤虫进行急性毒性试验.结果表明,培养5 d时对数生长初期单个藻细胞的溶血活性最强,达到1.80×10-7HU/cell,此后随培养时间的增加而逐渐降低;不同生长时期CMHK单位体积藻细胞的溶血活性与生长曲线基本吻合,对数生长末期最强,达到5.32 HU/L;Fe限制虽然能够显著抑制藻细胞的生长,但藻细胞的溶血活性明显增强,单个藻细胞的溶血活性达到8.37×10-7 HU/cell,为正常培养条件下溶血活性值（1.33×10-7 HU/cell）的6.3倍.另外,N限制可使藻细胞的溶血活性值达到6.67×10-7HU/cell;Mn和P限制也能够导致藻细胞溶血活性增强.不同生长期的CMHK对卤虫的毒性试验结果显示,对数生长末期的CMHK藻液对卤虫的毒性作用最强,卤虫暴露在藻密度为4.0×104cells/mL的藻液48 h后,死亡率达到77%,而对数生长初期藻液毒性最弱,48 h卤虫死亡率为20%.

英文摘要：

Hemolytic activity of Chattonella marina Hong Kong strain（CMHK） in different growth phase and nutrients structure was studied under laboratory conditions.According to the growth curve of CMHK,cells in culture were collected in logarithmic phase,stationary phase and decline phase,respectively,to determine hemolytic activity.Hemolytic activity of CHHK,which was cultured in different nutrients structure,was also determined.Furthermore,acute toxicity test of CMHK on Artemia salina was performed in early logarithmic phase（5 d）,late logarithmic phase（13 d）,stationary phase（15 d） and decline phase（17 d）.The results showed that the highest hemolytic activity was detected in early logarithmic phase.After 5 d culture,the hemolytic activity reached 1.80×10-7 HU/cell,then decreased gradually with culture time increasing.Hemolytic activity of total cells in culture in different growth phase coincided with the growth curve with the maximum reaching 5.32 HU/L in late logarithmic phase.Growth rate of CMHK was greatly depressed by Fe limitation while hemolytic activity per cell was enhanced to 8.37×10-7 HU/cell,nearly 6.5 times higher than that with no Fe limitation（1.33×10-7 HU/cell）.Similarly,N,Mn and P limitation could also increase the hemolytic activity of CMHK.Acute toxicity test of CMHK on A.salina showed that the maximum toxicity of the algae appeared at late logarithmic phase.It was found that death rate of A.sinica reached to 77% in 48 h when exposed to the algal culture at late logarithmic phase with algae density being 4.0×104 cells/mL,and.the lowest toxicity on A.sinica appeared in early logarithmic phase with death rate of 20%.

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