中文摘要：

建立了海洋水体与沉积物中30种光合色素的反相高效液相色谱检测方法。海水滤膜和沉积物分别采用95%甲醇和95%丙酮超声萃取。萃取液混合一定比例的超纯水后,采用Eclipse XDB C8反相柱进行分离,以乙腈（A）、甲醇（B）和四丁基羟胺-甲醇（C）混合液为流动相进行洗脱,检测波长为430,440和450nm,柱温为50℃。色谱分离梯度为：0min,100%C;22min,25%A,45%B和30%C;28～38min,70%A,20%B和10%C;38.1～40min,100%C。对23种已知浓度色素的线性关系、检出限和加标回收率进行分析。结果表明,相关系数范围为0.9972～0.9998,检出限范围为0.0305～0.7740ng,加标回收率范围为88.6%～103.3%。

英文摘要：

An analytical method was developed for the simultaneous determination of 30 photosynthetic pigments by reversed phase high performance liquid chromatography （RP-HPLC）. About 1 L of natural seawater was filtrated on a 0.45μm GF/F filter. The GF/F filters and marine sediments were extracted in 95% methanol and 95% acetone by ultrasonication, respectively. The separation of pigments extraction was performed on Eclipse XDB C8 column （150 minX4.6 mm, 3.5 p.m） at wavelength of 430, 440, 450 nm and at column temperature of 50 ℃. The mobile phase included three solvents, solvent A, solvent B and solvent B. Solvent A and B were acetonitrile and methanol, respectively. Solvent C was the mixed solution contained 70 ＂ 30 （V/V） methanol and 28 mmol/L aqueous tetrabutyl ammonium hydroxide-acetate （pH 6. 5）. The chromatographic process included 2 steps of gradient elution and 1 step of isocratic elution. From 0 to 22 min, the solvent A and B increased from 0 to 25% and 45%, respectively, and solvent C decreased from 100% to 30%, followed by gradient elution to 70% A, 20% B and 10% C for 6 min. Afterwards, the isocratic mode was performed for 10 rain and back to the initial conditions. The correlation coefficients for the 23 photosynthetic pigments, of which concentrations were known, ranged from 0. 9972 to 0. 9998, with detection limits from 0. 0305 ng to 0. 7740 ng, and recovery from 88.6% to 103.3%.