中文摘要：

目的：以＂女子以肝为先天＂的中医理论为基础,选用逍遥丸作为疏肝法的代表方,比较逍遥丸对排卵障碍模型大鼠子宫内膜雌激素受体（ER）、孕激素受体（PR）表达的影响。方法：雌性Wistar大鼠给予雷公藤多甙片,雌性SD大鼠注射丙酸睾丸酮,将2种排卵障碍成功模型大鼠随机分为模型组和治疗组（灌服逍遥丸）,给药5周,择动情前期,断头取血,检测血清雌二醇（E2）、孕酮（P）;取子宫,进行免疫组织化学染色,观察子宫内膜ER、PR的表达。结果：2种模型组均表现为血清E2、P值异常,子宫内膜ER、PR表达降低,差异均有统计学意义（P〈0.01或P〈0.05）;经逍遥丸治疗后,均有不同程度的改善,差异均有统计学意义（P〈0.05）;两治疗组血清E2及子宫内膜ER表达与正常组比较,差异无统计学意义。结论：逍遥丸可通过改善卵巢激素和子宫内膜ER、PR的表达治疗排卵障碍性疾病。

英文摘要：

Objective： Based on Traditional Chinese Medical theory about ＂the liver has important position in women＂,selected Xiaoyaowan as representative prescription of dispersing stagnated liver method,comparing the expression of endometrial ER,PR in ovulation obstacles rat models.Methods： The ovular model rats induced by giving TWP-chip to healthy female Wistar rats and injecting the female rats testosterone to healthy female SD rats.Each group rats were divided into model group and herbs-treated group（giving Xiaoyaowan）,5 weeks later,choosing estrogen prophase executed.Cut head and detected serum E2,P.Took the uterus,immunohistochemical staining,observed the expression of endometrial ER,PR.Results： Serum E2,P and endometrial ER,PR express of two model groups are abnormal.Endometrial ER,PR express abnormally,model groups are lesson compared with normal group,the differences are statistically significant（P〈0.01or P〈0.05）,the treatment groups were higher compared with model group,and had statistically significant difference（P〈0.05）.The expression of Serum E2 and ER of treatment groups recovered to normal level.Conclusion： Xiaoyaowan can treat ovulation barrier disease through improving the ovarian hormones and expression of endometrial ER and PR