中文摘要：

目的将调查在在 vitro 授精和胚胎转移(IVFet ) 经历的不肥沃的女人上劝慰肝治疗的效果并且探索它的机制。有管的不孕的 58 个女人被使随机化进二个组的方法：30 加释放 gonadotropin 与 Xiaoyao 在一个试验性的组对待粉末(Shugan ) 荷尔蒙类似物(GnRHa )/follicle-stimulating 荷尔蒙(FSH )/human chorionic gonadotropin (hCG ) 并且 28 在仅仅与 GnRHa/FSH/hCG 被对待的控制组。全部的 gonadotropin (Gn ) 剂量要求的、子宫内膜的厚度，卵母细胞数字，高质量的胚胎生产率和二个组的怀孕率被比较。在小囊的液体的生长区别 factor-9 (GDF-9 ) 的集中被西方的弄污检测，在 granulosa 房间的 GDF-9 mRNA 的表示用反向的抄写聚合酶链反应扩大被测量。在试验性的组，的结果 Gn 剂量在控制组是比那显著地低的；子宫内膜的厚度，高质量的胚胎生产和怀孕率显著地更高， GDF-9 mRNA 的表达式也比在控制组显著地高(所有 P < 0.05 ) 。结论 Shugan 处理能与管的不孕改进女人的怀孕率；它的机制是可能与在 granulosa 房间的 GDF-9 的增加的表示有关。

英文摘要：

OBJECTIVE： To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer（IVF-ET） and to explore its mechanism.METHODS： Fifty-eight women with tubal infertility were randomized into two groups： 30 in an experimental group treated with Xiaoyao powder（Shugan） plus gonadotropin-releasing hormone analog（GnRHa）/follicle-stimulating hormone（FSH）/human chorionic gonadotropin（hCG） and 28 in the control group who were treated with GnRHa/FSH/hCG only. The total gonadotropin（Gn） doses required, endometrial thickness, oocyte numbers,high quality embryo production rate and pregnancy rate of the two groups were compared. The concentration of growth differentiation factor-9（GDF-9） in follicular fluid was detected by western blotting and the expression of GDF-9 mRNA in granulosa cells was measured using reverse tran-scription-polymerase chain reaction amplification.RESULTS： In the experimental group, the Gn dose was significantly lower than that in the control group; the endometrial thickness, high quality embryo production and pregnancy rates were significantly higher and the expression of GDF-9 mRNA was also significantly higher than in the control group（all P0.05）.CONCLUSION： Shugan treatment can improve the pregnancy rate of women with tubal infertility; its mechanism is possibly related to the increased expression of GDF-9 in granulosa cells.