中文摘要：

以欧洲甜樱桃优良矮化砧木CAB-6p试管苗幼嫩叶片为试材，从基本培养基、激素配比、叶片生理状态和培养基中琼脂用量等方面对影响离体叶片再生的关键因素进行了研究。结果表明，CAB-6p试管苗幼嫩叶片以WPM为基本培养基再生效果最好，明显优于QL和DKW培养基，1／2MS培养基再生效果最差；最佳激素配比是BA2mg／L＋IAA2m玑，用IBA或NAA替代IAA出现愈伤组织生长量大但再生率低；CAB-6p试管苗顶部新发出合拢的幼嫩叶片再生能力最高，半展开的幼嫩叶片和完全展开的幼嫩叶片未能再生植株；用4．5g/L琼脂配制偏软的再生培养基明显有利于提高离体叶片的再生效率。通过以上几个方面的优化，建立CAB-6p试管苗幼嫩叶片高效离体再生技术体系，再生率可稳定地保持在90％左右，平均每叶再生4～5芽。

英文摘要：

Experiment was conducted with the young leaves in vitro of the sweet cherry rootstock CAB-6p for studying the effects of the different basic culture media, hormone, agar and physiological status of leaves on the adventitious shoots regeneration. The results showed that WPM medium was the optimal basic culture medium for the adventitious shoots regeneration and the regeneration rate was 90% with 3.8 shoots per leaf. Regeneration rate was 66% with 3.2 shoots per leaf in QL medium and 58% with 2.8 shoots per leaf in DKW medium. 1/2MS medium was the most unsuitable basic culture medium and regeneration rate was 8% with 1.0 shoots per leaf. The optimal hormone contents were BA 2 mg/L ＋ IAA 2 mg/L. In the culture media containing IBA or NAA, instead of IAA, the young leaves in vitro formatted more callus and regenerated little adventitious shoots. Only from the best young and folding leaves in vitro with the best physiological status, the adventitious shoots regeneration were the most effective and regeneration rate was 90% with 3.5 shoots per leaf. No adventitious shoot could be regenerated from semi-folding or expanding young leaves. Using safter and half gelling regeneration medium through decreasing agar content to 4.5 g/L was a very effective and significant method to improve adventitious shoot regeneration. Regeneration rate was 90% with 5.1 shoots per leaf in the medium with 4.5 g/L agar and 66% with 4.4 shoots per leaf in the medium with 6.0 g/L agar that was usually used in plant micro-propagation medium. In the high efficient regeneration system, regeneration rate was 90% or so and 4 to 5 shoots per leaf and the results were stable.